Special pretreatment liquid for rectal internal liquid samples and preparation method thereof

A pretreatment liquid and sample technology, applied in the direction of botanical equipment and methods, preparation of test samples, chemicals for biological control, etc., can solve problems affecting the accuracy of test results, etc., and achieve a wide range of pH applications, The effective dosage is small and the effect of ensuring accuracy

Active Publication Date: 2019-09-27
强安医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Mold growth will seriously affe

Method used

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  • Special pretreatment liquid for rectal internal liquid samples and preparation method thereof

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Embodiment 1

[0025] Embodiment 1 illustrates high-concentration pretreatment liquid of the present invention

[0026] 1. Prepare 1000ml of 0.5mol / L, pH7.4 phosphate buffer solution;

[0027] 2. Add 3.2 g of disodium edetate and 0.6 g of sodium lauryl sulfate to the phosphate buffer solution obtained in step 1, and heat to dissolve in a water bath at 57°C;

[0028] 3. Add 7ml of isobutanol to the solution prepared in step 2, stir well to dissolve it completely;

[0029] 4. Add 80 g of shell iodine and 1.5 ml of methylisothiazolinone into the solution prepared in step 3, and stir to mix them evenly.

Embodiment 2

[0031] Embodiment 2 illustrates the preferred pretreatment liquid of the present invention

[0032] 1. Prepare 1000ml of 0.5mol / L, pH7.4 phosphate buffer solution;

[0033] 2. Add 2.0 g of disodium edetate and 0.4 g of sodium lauryl sulfate to the phosphate buffer solution obtained in step 1, and heat to dissolve in a 60°C water bath;

[0034] 3. Add 4ml of isobutanol to the solution prepared in step 2, stir well to dissolve it completely;

[0035] 4. Add 65 g of shell iodine and 0.1 ml of methylisothiazolinone into the solution prepared in step 3, and stir to mix them evenly.

Embodiment 3

[0037] Embodiment 3 illustrates the low concentration pretreatment liquid of the present invention

[0038] 1. Prepare 1000ml of 0.5mol / L, pH7.4 phosphate buffer solution;

[0039] 2. Add 1.6 g of disodium edetate and 0.2 g of sodium lauryl sulfate to the phosphate buffer solution obtained in step 1, and heat to dissolve in a water bath at 58°C;

[0040] 3. Add 3ml of isobutanol to the solution prepared in step 2, stir well to dissolve it completely;

[0041] 4. Add 55 g of shell iodine and 0.05 ml of methylisothiazolinone into the solution prepared in step 3, and stir to mix them evenly.

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Abstract

The invention relates to special pretreatment liquid for rectal internal liquid samples with mold inhibition and cell disruption functions. The special pretreatment liquid comprises components of methylisothiazolinone, lauryl sodium sulfate, ethylenediamine tetraacetic acid disodium, isobutanol, chitosan-iodine complex and phosphate buffer. The invention also relates to a preparation method of the pretreatment liquid. The pretreatment liquid provided by the invention can provide a stable environment for the samples, due to the joint use of the methylisothiazolinone and the chitosan-iodine complex, microorganisms, especially molds, in a pretreatment process of the detection of the rectal internal liquid samples can be killed and the growth of which is inhibited, meanwhile, due to the superimposition of triple breaking functions of the lauryl sodium sulfate, the isobutanol and distilled water on type cells, the type cells in the samples are broken to release intracellular substances, thereby preventing the intracellular substances in the samples from solidifying and precipitating, and ensuring the accuracy of sample detection.

Description

technical field [0001] The invention belongs to the field of medical supplies, in particular to a special pretreatment solution for rectal fluid samples and a preparation method thereof. Background technique [0002] Rectal fluid is a kind of human body sample used in medical clinical testing, which can be used for biochemical analysis and microbial detection of potassium, sodium, chlorine, pH value, heme protein, etc. Rectal fluid contains intestinal secretions, pathological exudates, residual food waste, shaped cells (red blood cells, white blood cells, plants, animals, microbial cells, etc.), and there may be residual feces, especially loose stools, etc. Intestinal contents. Due to the particularity of the environment in the rectum, there are many bacteria, molds, and parasites. The pre-treatment solution is a component of in vitro diagnostic reagents and requires a certain period of validity, generally 1 to 2 years. During the storage of liquid-based reagents, molds o...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30A01N25/30A01N43/80A01N43/16A01N59/12A01P3/00A01P1/00
CPCA01N25/30A01N43/16A01N43/80A01N59/12G01N1/28G01N1/30
Inventor 张野杨新财
Owner 强安医疗器械有限公司
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