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Dendrobium candidum seed culture medium with natural plant hormones

Dendrobium officinale, natural plant technology, applied in plant regeneration, botany equipment and methods, horticulture, etc., can solve the problems of environmental pollution of chemically synthesized hormones, and achieve the effect of vigorous root growth

Inactive Publication Date: 2019-10-01
CHENGDU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the germination rate of Dendrobium officinale seeds is increased by adding chemically synthesized hormones and other additives to the basic medium, but chemically synthesized hormones are likely to cause environmental pollution.

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0012] The invention provides a Dendrobium officinale seed culture medium added with natural plant hormones. The preparation method comprises: taking 1 / 2MS as the base medium, adding mung bean sprout grinding liquid with a concentration of 50-150g / L, and the concentration is 30g / L sucrose, agar with a concentration of 7g / L, sterilized at pH 5.8, 121°C for 20min.

[0013] Further, the concentration of mung bean sprouts grinding liquid is 50g / L.

[0014] Further, it also includes adding potato grinding liquid with a concentration of 20-150g / L. And when the concentration of mung bean sprouts grinding liquid is 150g / L, the effect is the best when the concentration of potato grinding liquid is 100g / L.

[0015] It should be noted that, in the present application, the preparation method of the mung bean sprout grinding liquid comprises: weighing the mung bean sprouts, washing with clear water, filtering and drying, cutting into small sections, putting them into a mixing cup of a jui...

Embodiment 1

[0019] The present embodiment provides a kind of Dendrobium candidum seed culture medium that adds natural plant hormone, and its preparation method comprises: take 1 / 2MS as basic culture medium, add the mung bean sprout grinding liquid that concentration is 50g / L, concentration is 30g / L Sucrose, agar with a concentration of 7g / L, sterilized at pH 5.8 at 121°C for 20min.

Embodiment 2

[0021] This embodiment provides a kind of Dendrobium officinale seed culture medium that adds natural plant hormone, and its preparation method comprises: take 1 / 2MS as basic culture medium, add the mung bean sprout grinding liquid that concentration is 50g / L, concentration is 20g / L Potato grinding fluid, 30 g / L sucrose, 7 g / L agar, sterilized at 121°C for 20 min at pH 5.8.

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Abstract

The invention belongs to the technical field of culture media and provides a Dendrobium candidum seed culture medium with natural plant hormones. A preparation method of the Dendrobium candidum seed culture medium with natural plant hormones comprises adding mung bean sprout ground pulp having a concentration of 50-150 g / L, cane sugar having a concentration of 30 g / L and agar having a concentration of 7 g / L into 1 / 2MS acting as a basic medium, and disinfecting under pH of 5.8 at 121 DEG C for 20 min. The Dendrobium candidum seed culture medium with natural plant hormones herein helps lessen the environmental damage due to the emission of chemical hormones; the concern of people with artificial synthetic hormones used in manual planting of Dendrobium candidum can be eliminated; rooting rateof Dendrobium candidum seedlings can be increased greatly by the use of the medium of the invention.

Description

technical field [0001] The invention belongs to the technical field of seed culture medium, in particular to a dendrobium officinale seed culture medium added with natural plant hormones. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium officinale, known as the first of the "nine Chinese immortal grasses". It is sweet in taste and slightly cold in nature. It is used for fever, dry mouth, polydipsia, insufficient stomach yin, retching due to lack of food, persistent asthenia heat after illness, hyperactivity of fire due to yin deficiency, bone steaming, labor heat, blurred eyesight, musculoskeletal Atrophy soft. The main components of Dendrobium officinale are Dendrobium polysaccharide and dendrobine, which have the functions of nourishing yin, benefiting stomach, promoting body fluid and quenching thirst, moistening lung and relieving cough, strengthening and enhancing immune activity. In recent years,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 蒋伟车莹谌菊
Owner CHENGDU NORMAL UNIV
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