A kind of culture medium and culture method for culturing brain tumor cells in vitro
An in vitro culture and brain tumor technology, applied in the direction of tumor/cancer cells, culture process, tissue culture, etc., can solve the problems of drug screening for medulloblastoma
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[0048] Experimental animals: conditional knockout transgenic mice Ptch1fl / fl Mice, Math1-Cre Mice, CB17 / SCIDMice, purchased from Jackson Laboratory, all experimental animals are in line with SPF level (Specific Pathogen Free, no specific pathogens), all animals are treated All operations have been legally and effectively approved; immunodeficiency mice (CB17 / SCID Mice), intracranial injection transplantation using a mouse brain stereotaxic instrument (KOPF, Nikon SMZ1000) and a 5ul micro-injector (Shanghai Gaoge).
[0049] Cell culture:
[0050] (1) The primary medulloblastoma cells were all extracted from the cerebellum of the above-mentioned conditional knockout adult mice, and the mouse cerebellar medulloblastoma tissue was digested with digestive juice, and the digestive juice components (10ml): 100U of papain ( Papain, Worthington Biochemical, LS003126), 2 mg of cysteine (L-cysteine, Sigma) and 2500 U of DNase (DNase, Sigma, D4527), digested in a water bath at 37°C for ...
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