Method for preparing compounded brassinolide plant growth regulator easy to degrade

A technology for plant growth regulator and brassinolide, which is applied in the field of preparation of easily degradable compound brassinolide plant growth regulator, and can solve the problems of high cost, high preparation cost, low yield and purity, etc. , to achieve the effect of low cost and simple preparation steps

Inactive Publication Date: 2016-08-10
袁春华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: A large number of organic solvents such as carbon tetrachloride and chloroform are used for extraction in the current preparation method of brassinolide, the preparation process has great environmental pollution, high preparation cost, and the organic solvent is easy to remain after use, and It is prepared by extracting plants, etc., which has the disadvantages of high cost, low yield and purity. It provides a method of mixing rapeseed pollen and agaricus in hydrochloric acid solution to soak in hydrochloric acid solution to prepare pretreated mixed powder for later use, and then mix it with nutrient solution and discarded edible fungi. The mycelia suspension obtained from the culture medium is mixed and fermented, extruded to obtain an extruded liquid, mixed with ethyl acetate, centrifuged, concentrated to obtain a base liquid of an easily degradable compound brassinolide plant growth regulator, and mixed with enzymatic hydrolysis and filtration The prepared filtrate is reacted with potassium sodium tartrate, and the filtrate is discharged and filtered to obtain a method for easily degradable compound brassinolide plant growth regulator

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] First, according to the mass ratio of 3:1, put the rapeseed pollen and agaric cauliflower into a mixer and mix evenly, soak them in a hydrochloric acid solution with a mass fraction of 5%, filter after standing for 30 minutes, and put the filtered matter in an oven , dried at 60°C for 2 hours, then put the dried filtrate into a pulverizer to pulverize, pass through a 200-mesh sieve, and obtain a pretreated mixed powder for subsequent use; in parts by weight, take 50 parts of peptone, 15 parts of agar, 2 1 part of sodium α-naphthalene acetate, 30 parts of distilled water and 10 parts of sucrose, and mix them evenly to obtain a nutrient solution. Take the discarded edible fungus culture medium and soak it in the nutrient solution, let it stand for 32 minutes, take out the discarded edible fungus culture medium and put it in a container. Seal the container, keep the temperature in the container at 30°C, let it stand for 1 day, then take out the waste edible fungus culture m...

example 2

[0020]First, according to the mass ratio of 3:1, put the rapeseed pollen and agarose cauliflower into a mixer and mix evenly, soak them in a hydrochloric acid solution with a mass fraction of 5%, filter after standing for 35 minutes, and put the filtered matter in an oven , dried at 60°C for 3 hours, then put the dried filtrate into a pulverizer to pulverize, pass through a 200-mesh sieve, and obtain a pretreated mixed powder for subsequent use; in parts by weight, take 53 parts of peptone, 18 parts of agar, 3 parts of agar 1 part of sodium α-naphthalene acetate, 32 parts of distilled water and 13 parts of sucrose, mixed evenly to obtain a nutrient solution, soaked in the nutrient solution with the discarded edible fungus culture medium, and allowed to stand for 34 minutes, then took out the discarded edible fungus culture medium and put it in a container, Seal the container, keep the temperature in the container at 33°C, let it stand for 2 days, then take out the waste edible ...

example 3

[0023] First, according to the mass ratio of 3:1, put the rapeseed pollen and agaric cauliflower into a mixer and mix evenly, then soak them in a hydrochloric acid solution with a mass fraction of 5%, filter after standing for 40min, and put the filtered matter in an oven , dried at 60°C for 3 hours, then put the dried filtrate into a pulverizer for pulverization, and passed through a 200-mesh sieve to obtain a pretreated mixed powder for subsequent use; in parts by weight, 55 parts of peptone, 20 parts of agar, 3 parts of agar 1 part of sodium α-naphthalene acetate, 35 parts of distilled water and 15 parts of sucrose, and mix them evenly to obtain a nutrient solution. Take the waste edible fungus culture medium and soak it in the nutrient solution, let it stand for 35 minutes, take out the waste edible fungus culture medium and put it in a container. Seal the container, keep the temperature in the container at 35°C, let it stand for 2 days, then take out the waste edible fungu...

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Abstract

The invention discloses a method for preparing a compounded brassinolide plant growth regulator easy to degrade, and belongs to the technical field of preparation of plant growth regulators .Rape pollen and agar weeds are mixed in a hydrochloric acid solution to be soaked, pretreatment mixed powder is prepared for use, a hypha suspension liquid is prepared from the pretreatment mixed powder, a nutrient solution and a waste edible mushroom culture medium for mixed fermentation, an extrusion solution is obtained through extruding and mixed with ethyl acetate, and a base solution of the compounded brassinolide plant growth regulator easy to degrade is obtained through centrifugation and concentration; the base solution, filterable matter obtained through enzymolysis and filtering and sodium potassium tartrate tetrahydrate are mixed and react, discharging and filtering are carried out to obtain filterable matter, and the compounded brassinolide plant growth regulator easy to degrade is prepared .The method has the advantages that the preparation steps are simple, no organic solvent is needed, the preparation process is free of pollution to the environment, and the cost is lower than that of other methods by 18% or above; the product yield is higher than that of other methods by 13.2% or above, and the purity is higher than that of other products by 20.3% or above.

Description

technical field [0001] The invention relates to a preparation method of an easily degradable compound brassinolide plant growth regulator, belonging to the technical field of plant growth regulator preparation. Background technique [0002] Brassinolide is a new type of green and environment-friendly plant growth regulator, which is treated by soaking seeds with appropriate concentration of brassinolide and spraying stems and leaves. It can promote the growth of vegetables, melons, fruits and other crops, improve quality, increase yield, bright color and thicker leaves. It can also advance the picking time of tea leaves, and can also make melons and fruits have higher sugar content, larger individuals, higher yields, and better storage resistance. Plant hormones are harmless to humans and animals, and the normal dosage is very safe and effective. Natural brassinolide can be widely used in various economic crops, generally can increase the yield by 5-10%, and the high one c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N43/22A01N37/10A01P21/00
CPCA01N37/10A01N43/22A01N63/10A01N2300/00
Inventor 袁春华盛海丰宋奇
Owner 袁春华
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