Preparation method of controlled release hydrogel used for tumor postoperative chemotherapy magnetic-thermal and photo-thermal combined treatment
A combined therapy and hydrogel technology, which is applied in the field of controlled-release hydrogel preparation, can solve the problems of inability to remove tumors, trauma and complications of patients, and normal cell damage, and achieve easy storage, reduced dosage, Effect of Good Controlled Release Properties
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0036] 1) Add 1 g of carboxymethyl chitosan to 47 mL of MES buffer and stir for 2 h;
[0037] 2) Dissolve 85mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 51mg of N-hydroxysuccinimide in 1mL of MES buffer respectively;
[0038] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1 h, during which the pH of the solution is maintained at 5 with hydrochloric acid or sodium hydroxide;
[0039] 4) Finally, add 550 mg of dopamine to the solution obtained in step 3), and then react for 12 hours under stirring, and maintain the pH of the solution at 5 with hydrochloric acid or sodium hydroxide during stirring;
[0040] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 2 days, then frozen in an ultr...
Embodiment 2
[0049] 1) Add 1.25g carboxymethyl chitosan to 47.5mL MES buffer, stir for 2h;
[0050] 2) Dissolve 87.5 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 53 mg of N-hydroxysuccinimide in 1.25 mL of MES buffer respectively;
[0051] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1.25h, and maintain the pH of the solution at 5 with hydrochloric acid or sodium hydroxide during stirring;
[0052] 4) Add 570 mg of dopamine to the solution obtained in step 3), and react under stirring for 14 hours. During the stirring, use hydrochloric acid or sodium hydroxide to maintain the pH of the solution at 5;
[0053] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 2 days, then frozen in an ultra-low ...
Embodiment 3
[0062] 1) Add 1.5g carboxymethyl chitosan to 48mL MES buffer, stir for 2h;
[0063] 2) Dissolve 90mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 55mg of N-hydroxysuccinimide in 1.5mL of MES buffer respectively;
[0064] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1.5h, and maintain the pH of the solution at 6 with hydrochloric acid or sodium hydroxide during stirring;
[0065] 4) Add 600 mg of dopamine to the solution obtained in step 3), and then react for 16 hours under stirring, and maintain the pH of the solution at 6 with hydrochloric acid or sodium hydroxide during stirring;
[0066] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 3 days, then frozen in an ultra-low temperat...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com