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Multifunctional liposome based on hydrogen peroxide response and its preparation method and application

A technology of hydrogen peroxide and liposome, which is applied in the field of nanomaterials, can solve the problems of lack of application of nanometer drug-carrying systems, achieve the effects of optimizing imaging capabilities and drug treatment effects, prolonging circulation time, and avoiding decomposition

Active Publication Date: 2021-07-09
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the field of atherosclerotic diseases, the application of nano-drug delivery system integrating diagnosis and treatment is relatively lacking, which needs to be supplemented and improved urgently.

Method used

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  • Multifunctional liposome based on hydrogen peroxide response and its preparation method and application
  • Multifunctional liposome based on hydrogen peroxide response and its preparation method and application
  • Multifunctional liposome based on hydrogen peroxide response and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the synthesis of PEG-PPS amphiphilic polymer

[0043] Add 50mL of anhydrous chloroform into a 100mL round bottom flask, weigh 7.50g of mPEG 2000 dissolve in it. Place in an ice-salt bath, add 0.80 mL of triethylamine, 45 mg of DMAP and 1.00 g of p-toluenesulfonyl chloride and stir for 5 min. Then the reaction device was placed at room temperature for 20 h. After the reaction, the product was concentrated by evaporation and precipitated with 500 mL of diethyl ether. After filtration, it was dried in a vacuum oven for 48 hours and then weighed to obtain 6.48 g of the product. Weigh 5.00 g of the above product and dissolve it in 12 mL of methanol, and add 3.42 g of potassium thioacetate and 16 mL of triethylamine to react for 36 h. After the reaction was completed, the solid was re-dissolved uniformly in chloroform, extracted with saturated sodium bicarbonate and sodium chloride solution, and finally dried with anhydrous magnesium sulfate for 24 hours. A...

Embodiment 2

[0044] Embodiment 2: Preparation of PtdSer@PEG-PPS nanoliposomes

[0045] Accurately weigh 20 mg of phosphatidylserine and 5 mg of PEG-PPS in an eggplant-shaped flask containing 5 mL of chloroform, and remove the organic solvent by rotary evaporation at 40 °C to form a thin film at the bottom of the flask. After drying in a vacuum oven for 12 hours, add 10 mL of PBS buffer solution with pH=7.4, shake and hydrate at 55°C for 45 minutes, and then filter through a 0.45 μm, 0.μm22 microporous membrane to obtain the bimodal probe lipid body.

Embodiment 3

[0046] Example 3: FITC-SiO 2 @Fe 3 o 4 Preparation of (FSF) bimodal probes

[0047] Put 12 mL of absolute ethanol and 50 μL of 3-aminopropyltriethylsilane in a 100 mL round bottom flask, and dissolve 10 mg of FITC into the above solution, and dissolve under ultrasonic conditions for 10 min. Mix 15mL of deionized water, 80mL of absolute ethanol, and 1mL of 25% ammonia water in a 250mL round bottom flask, and 20mg of SiO 2 @Fe 3 o 4 Nanoparticles were added into it, and mechanically stirred in a dark place after being uniformly dispersed, and 1 mL of tetraethyl orthosilicate was added thereto, and then the pre-prepared FITC solution was slowly added dropwise, and reacted for 10 h after the dropwise addition was completed. After the reaction, separated by a strong magnetic field, washed several times with distilled water and absolute ethanol, and dried in a vacuum oven for 3 days to obtain FITC-SiO2@Fe3O4(FSF). figure 2 Mid-infrared characterization demonstrates the succes...

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Abstract

The hydrogen peroxide-responsive multifunctional liposome and its preparation method and application of the present invention belong to the technical field of nanomaterials. The hydrogen peroxide-responsive multifunctional liposome is composed of phosphatidylserine and double-block polymerization Polyethylene glycol-polypropylene sulfide is jointly constituted; its preparation method comprises the steps of preparation of PEG-PPS and preparation of hydrogen peroxide-responsive multifunctional liposomes. The bimodal probe FITC-SiO was coated with the described hydrogen peroxide-responsive multifunctional liposome 2 @Fe 3 o 4 It can be applied to in vivo imaging; coating anti-atherosclerotic drugs with hydrogen peroxide-responsive multifunctional liposomes can be used for drug release in vivo. In the present invention, biodegradable polyethylene glycol is conjugated to the outer surface of liposomes to produce stealth carriers to prevent immune clearance; at the same time, traditional polyethylene glycol is specially modified to retain biocompatibility Capable of releasing imaging agents or drugs in response to hydrogen peroxide.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and specifically relates to the preparation and application of a multifunctional liposome with hydrogen peroxide response, targeted nuclear magnetic fluorescence imaging and drug release capabilities. Background technique [0002] Atherosclerosis is a common cardiovascular and cerebrovascular disease. As a chronic inflammatory disease, atherosclerosis is latent and hidden in the early stage, and the symptoms are not obvious, but it can eventually lead to high-risk diseases such as myocardial infarction and stroke. In terms of treatment, atherosclerotic disease can only be cured by stents, surgery and other means in the later stage. In the early stage, oral drug therapy was more common, but this method of treatment has problems such as low drug utilization rate, slow effect, and serious side effects. In addition, atherosclerosis lacks the pH-sensitive ability of traditional diseases, and a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/54A61K49/18A61K49/10A61K49/00A61P9/10B82Y5/00B82Y20/00B82Y40/00
CPCA61K49/0002A61K49/0021A61K49/0052A61K49/0086A61K49/10A61K49/1812A61K47/544A61K47/6915A61P9/10B82Y5/00B82Y20/00B82Y40/00
Inventor 李亚鹏李少静于艳辉朱焓毓姚顺雨沈美丽武小东
Owner JILIN UNIV
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