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Bone repair material containing nano hydroxylapatite/collagen particle and preparation thereof

A nano-hydroxyapatite and bone repair technology, applied in medical science, prostheses, etc., can solve the problems of long-term effect observation and inconvenient application, and achieve no cytotoxicity, easy processing, and convenient operation Effect

Active Publication Date: 2011-11-23
BEIJING ALLGENS MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2005, Hoemann et al. (Hoemarm CD, Sun J, Buschmann MD, et al. Tissue engineering of cartilage using an injectable and adhesive chitosan-based-cell delivery vehicle. Osteoarthritis Cartilage, 2005, 13: 318-329) improved chitosan Sugar / GP system, with chitosan, β-sodium glycerophosphate, and glucosamine as raw materials, thermosensitive chitosan hydrogel was also prepared by adjusting the temperature and pH value of the solution, and mixed with ethyl cellulose Suspended primary calf chondrocytes were injected subcutaneously into the back of nude mice to construct tissue-engineered cartilage. At the same time, chitosan hydrogel without cells was directly injected into the joint defect of rabbits for defect repair experiments. One week after operation Observation found that the implantation of this material has a certain repairing effect on cartilage defects, but no long-term observation of the effect
However, the pure mineralized polypeptide material is in the form of particles, and it is not a bone repair material that can be injected in situ, and its application is inconvenient.

Method used

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  • Bone repair material containing nano hydroxylapatite/collagen particle and preparation thereof
  • Bone repair material containing nano hydroxylapatite/collagen particle and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] It contains 2 parts by weight of nano-hydroxyapatite / collagen microparticles, 1 part by weight of chitosan and 2.5 parts by weight of sodium beta-glycerophosphate.

[0027] 200mg chitosan (degree of deacetylation 99%) was dissolved in 9ml hydrochloric acid (0.1M), stirred and dissolved until clear and placed in a water bath at 4 degrees Celsius; GP500mg was dissolved in 1ml distilled water to obtain a clear and homogeneous GP solution ( 50%, w / v); With 1M sodium bicarbonate solution, the pH value of the chitosan solution was adjusted to 4, and the GP solution was carefully dropped into the above-mentioned chitosan solution, and the pH value was adjusted to 7.15, and 400 mg of nano-hydroxyl Apatite / collagen microparticles (n HAC) were added to the above solution. Inject the blended solution into a 15mm×5mm×5mm mold and place it in a 37-degree incubator to form gel A in situ.

[0028] 200mg chitosan (degree of deacetylation 99%) was dissolved in 9ml hydrochloric acid (0....

Embodiment 2

[0039] It contains 1 weight part of nano-hydroxyapatite / collagen particles, 1 weight part of chitosan and 3 weight parts of beta-glycerophosphate sodium.

[0040] 200mg chitosan (80% degree of deacetylation) was dissolved in 9ml hydrochloric acid (0.1M), stirred and dissolved until clear and placed in a water bath at 4 degrees Celsius; GP600mg was dissolved in 1ml distilled water to obtain a clear and homogeneous GP solution ( 56%, w / v); With the KOH solution of 1M, the pH value of the chitosan solution is adjusted to 5, and the GP solution is carefully dripped into the above-mentioned chitosan solution, and the pH value is adjusted to 7.2, and 200 mg of nano-hydroxyapatite Stone / collagen microparticles (nHAC) were added to the above solution. Inject the solution into a 15mm×5mm×5mm mold, place it in a 37-degree incubator, and form gel C in situ within 5 minutes.

[0041] According to the patent 200610144009.5, a preparation method of a calcium alginate-based injectable in-si...

Embodiment 3

[0050] It contains 1.5 parts by weight of nano-hydroxyapatite / collagen particles, 1 part by weight of chitosan and 2 parts by weight of sodium beta-glycerophosphate.

[0051] 200mg chitosan (90% degree of deacetylation) was dissolved in 9ml hydrochloric acid (0.1M), stirred and dissolved until clear and placed in a water bath at 4 degrees Celsius; GP400mg was dissolved in 1ml distilled water to obtain a clear and homogeneous GP solution ( 50%, w / v); With the sodium phosphate solution of 1M, the pH value of the chitosan solution is adjusted to 5, the GP solution is carefully dripped into the above-mentioned chitosan solution, and the pH value is adjusted to 7.4, and the 300mg nanometer hydroxyl phosphorus Limestone / collagen microparticles (nHAC) were added to the above solution. The blended solution was placed in an environment of 37 degrees, and a gel could be formed in situ within 5 minutes.

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Abstract

The invention relates to a bone repair material containing nano hydroxyapatite / collagen particles, and a preparation method thereof. The invention provides a bone repair material which comprises: 1 to 2 parts by weight of the nano hydroxyapatite / collagen particles, 1 part by weight of chitosan and 2 to 3 parts by weight of Beta-sodium glycerophosphate (which is supplemented by the weight proportion of the components). The invention also provides a preparation method of the bone repair material; the bone repair material is placed at 37 DEG C or injected into human body, thus forming in-situ gelatin. The bone repair material provided by the invention not only can retain the characteristics of modeled natural bone structure, but also ensures the new performance of syringeability of the material and has temperature sensitivity, thus being applicable to minimal invasive surgery and convenient for clinical application.

Description

technical field [0001] The invention relates to an injectable bone repair material containing nano-hydroxyapatite / collagen particles and a preparation method thereof. Background technique [0002] Chitosan is a natural biological polysaccharide with a wide range of sources, no cytotoxicity, good biocompatibility and no antigenicity, absorbable and easy to process. It has been used in artificial skin, absorbable sutures, hemostatic sea Cotton, anti-adhesion agent and drug sustained-release capsules, etc. [0003] According to the interaction force between chitosan solution ions, in 2000, Chenite et al. (AChenite, C Chaput, D Wang, et al.Novel injectable neutral solutions of chitosan from biodegradable gels in situ. 2161) developed a thermosensitive chitosan hydrogel. The specific method is to add polyalcohol salts such as β-glycerophosphate sodium (GP) to the chitosan aqueous solution. Under the synergy of hydrogen bonds, electrostatic interactions and hydrophobic interacti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/50
Inventor 冯庆玲黄智崔福斋胡堃
Owner BEIJING ALLGENS MEDICAL SCI & TECH
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