A method for denitrification of wastewater discharged from the production process of hydroxyethyl cellulose
A technology of hydroxyethyl cellulose, production process, applied in the field of biological treatment of sewage treatment in environmental engineering
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Embodiment 1
[0031] The nitrogen-removing bacteria agent used in the present invention can be obtained by using a suitable medium to amplify the corresponding strains and mix them, or use a suitable medium to amplify the cultures after mixing the strains, which is well known to those skilled in the art. , such as LB medium, its components can be tryptone 10g / L, yeast powder 5g / L, NaCl 5g / L, pH7.2.
[0032] The denitrification bacteria agent includes Aeromonas ( Aeromonas sp. ) PR-DN1, Alcaligenes ( Alcaligenes sp. ) PR-DN2, Acinetobacter ( Acinetobacter sp. ) PR-DN3, Bacillus cereus ( Bacillus cereus. ) PR-DN4, microbacteria ( Exiguobacterium sp. ) PR-DN5; 9 cfu / g-4×10 9 cfu / g.
[0033] The preparation method of the denitrification bacteria agent is as follows: inoculate the bacteria involved in the treatment of hydroxyethyl cellulose production wastewater into LB medium (the volume of the bacteria is 2-5% of the volume of the LB medium), and inoculate them at 30 Under the con...
Embodiment 2
[0035] Composition of microbial denitrification bacteria:
[0036] Aeromonas ( Aeromonas sp. ) PR-DN1: 5×10 8 cfu / mL
[0037] Alcaligenes ( Alcaligenes sp. ) PR-DN2: 2.5×10 8 cfu / mL
[0038] Acinetobacter ( Acinetobacter sp. ) PR-DN3: 2.5×10 8 cfu / mL
[0039] Bacillus cereus ( Bacillus cereus. ) PR-DN4: 5×10 8 cfu / mL
[0040] microbacteria ( Exiguobacterium sp. ) PR-DN5: 2.5×10 8 cfu / mL
[0041] Total number of live bacteria: 1.75×10 9 cfu / mL
[0042] The denitrification bacterial agent was prepared according to the method of Example 1. Nitrogen-containing wastewater from a certain hydroxyethyl cellulose production is treated by A / O process. NH in wastewater 3 -N concentration is 20mg / L, NO 3 - -N concentration is 390mg / L, NO 2 - -N concentration is 5mg / L, COD Cr The concentration is 1000 mg / L. The treated wastewater cannot be discharged up to the standard. Add the denitrification bacteria agent prepared by the method of Example 1 into the sewage tr...
Embodiment 4
[0053] Composition of microbial denitrification bacteria:
[0054] Aeromonas ( Aeromonas sp. ) PR-DN1: 9×10 8 cfu / mL
[0055] Alcaligenes ( Alcaligenes sp. ) PR-DN2: 4×10 8 cfu / mL
[0056] Acinetobacter ( Acinetobacter sp. ) PR-DN3: 4×10 8 cfu / mL
[0057] Bacillus cereus ( Bacillus cereus. ) PR-DN4: 5×10 8 cfu / mL
[0058] microbacteria ( Exiguobacterium sp. ) PR-DN5: 3×10 8 cfu / mL
[0059] Total number of live bacteria: 2.5×10 9 cfu / mL
[0060] The method of Example 1 was used to prepare the denitrification bacteria agent. NH in Nitrogenous Wastewater from Certain Hydroxyethyl Cellulose Production 3 -N concentration is 11mg / L, NO 3 - -N concentration is 600mg / L, NO 2 - -N concentration is 12mg / L, COD Cr The concentration is 1500 mg / L. The treated wastewater cannot be discharged up to the standard. Add the denitrification bacteria agent prepared by the method of Example 1 into the sewage treatment system. During the dosing process, the temperature in...
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