Method for synthesizing pRNA-3WJ-siLNAgapmer-aptamer

An automated synthesis and gene-targeting technology, applied in the field of molecular biology, can solve the problems of low treatment success rate, achieve the effect of inhibiting growth and survival, and improving treatment success rate

Pending Publication Date: 2019-10-11
GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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Problems solved by technology

[0004] This application provides a method for synthesizing pRNA-3WJ-siLNAgapmer-aptamer, which solves the probl

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  • Method for synthesizing pRNA-3WJ-siLNAgapmer-aptamer

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Embodiment Construction

[0026] In order to enable those skilled in the art to better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings.

[0027] The core of this application is to provide a method for synthesizing pRNA-3WJ-siLNAgapmer-aptamer, which can solve the problem of how to overcome the low treatment success rate in the treatment of spinal tuberculosis.

[0028] figure 1 A flow chart of a method for synthesizing the nano-targeted gene pRNA-3WJ-siLNAgapmer-aptamer provided by the embodiments of the present invention, such as figure 1 As shown, the method includes the following steps:

[0029] S101: Packaging pRNA with phage Phi29 with "three-way junction" domain 3WJ, using pRNA-3WJ as a gene carrier;

[0030] S102: chimera small interfering LNA at one end of 3WJ;

[0031] S103: At the other end of 3WJ, an RNA aptamer c...

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Abstract

The invention discloses a method for synthesizing a nano-targeting gene pRNA-3WJ-siLNAgapmer-aptamer. Firstly, pRNA is packaged with a bacteriophage Phi29 with a 'three-way junction' structural domain3WJ, and pRNA-3WJ is taken as a gene vector; then small interfering LNA is in chimerism in one end of the 3WJ; an RNA aptamer of a CD40 molecule capable of being in targeted combination with a CD40 cell is in chimerism in the other end of the 3WJ to synthesize the nano-targeting gene pRNA-3WJ-siLNAgapmer-aptamer, wherein locked nucleic acid siLNAgapmer corresponds to a tuberculosis gene Mce4, andthe aptamer corresponds to CD40. The nano-targeting gene pRNA-3WJ-siLNAgapmer-aptamer can be targeted into a lesion, effectively inhibit the growth and survival of mycobacterium tuberculosis, and hasa high treatment success rate.

Description

technical field [0001] The application relates to the field of molecular biology, in particular to a method for synthesizing pRNA-3WJ-siLNAgapmer-aptamer. Background technique [0002] The existing anti-tuberculosis drugs are prone to drug resistance, and the concentration of drugs in spinal tuberculosis lesions is low due to reasons such as spinal tuberculosis hardening wall, which is prone to failure of spinal tuberculosis treatment. [0003] It can be seen that how to overcome the problem of low treatment success rate when treating spinal tuberculosis is a problem to be solved urgently by those skilled in the art. Contents of the invention [0004] This application provides a method for synthesizing pRNA-3WJ-siLNAgapmer-aptamer, which solves the problem of how to overcome the low treatment success rate in the treatment of spinal tuberculosis in the prior art. [0005] In order to solve the above-mentioned technical problems, the application provides a method for synthe...

Claims

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Application Information

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IPC IPC(8): C12N15/87A61K31/7088A61K47/54A61P31/06
CPCC12N15/87A61K31/7088A61K47/549A61P31/06
Inventor 王自立尹虎权樊宏杰雷建
Owner GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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