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Application of clomifene citrate to anti-mycobacterium tuberculosis medicines

A technology of clomiphene citrate and mycobacterium tuberculosis, applied in the field of bioengineering, can solve the problems of slow research and development of anti-tuberculosis drugs, cross-resistance, lack of effective preventive vaccines, etc., and achieve enhanced cell killing of tuberculosis, good The effect of developing value

Inactive Publication Date: 2019-10-22
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Due to the lack of effective preventive vaccines, lack of effective diagnostic techniques, and the occurrence of drug-resistant and refractory tuberculosis, the prevention and control of tuberculosis is still an important scientific problem at present, and the first-line anti-tuberculosis drugs used today are all used more than 40 years ago. Research and development, the development of new and effective anti-tuberculosis drugs is slow, and these drugs directly act on the tuberculosis itself, and there is always a risk of cross-drug resistance

Method used

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  • Application of clomifene citrate to anti-mycobacterium tuberculosis medicines
  • Application of clomifene citrate to anti-mycobacterium tuberculosis medicines
  • Application of clomifene citrate to anti-mycobacterium tuberculosis medicines

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1. Differentiation of U937 macrophages (No. BNCC100967): Suspended U937 macrophages were cultured in RPMI-1640 medium containing 10% FBS, and placed at a temperature of 37°C, containing 5% CO 2 Cultured in a cell incubator, added PMA with a final concentration of 20ng / ml, cultured overnight, differentiated into macrophages, washed twice with PBS, digested with trypsin, resuspended, added to a 96-well cell culture plate , so that the number of cells per well is 1x104;

[0022] 2. Determination of cell survival rate: After the above-mentioned U937 macrophages were cultured for 24 hours, different concentrations of drugs were added to make the final drug concentrations 100uM, 10uM, and 1uM. The experiment was repeated three times for each concentration, and DMSO was used as a parallel control experiment. In the control group, DMSO with the same volume as the drug was added, and the experiment was repeated three times. After 48 hours, 10ul of WST-1 solution was added to eac...

Embodiment 2

[0028] 1. Differentiation of U937 macrophages: Suspended U937 macrophages were cultured in RPMI-1640 medium containing 10% FBS, and placed at a temperature of 37°C, containing 5% CO 2 Cultured in a cell incubator, added PMA with a final concentration of 20ng / ml, cultured overnight, differentiated into macrophages, washed twice with PBS, digested with trypsin, resuspended, added to a 96-well cell culture plate , so that the number of cells per well is 1x104;

[0029] 2. Determination of intracellular bactericidal activity: After the above-mentioned U937 macrophages were cultured for 24 hours, clomiphene citrate was added to make the final concentration 10uM, and the experiment was repeated three times for each concentration, using DMSO as a parallel control experiment, and the control group Add DMSO with the same volume as the drug, repeat the experiment 3 times, remove the culture medium after 4 hours, wash twice with PBS, add fresh culture medium for 72 hours, add lysate, spr...

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Abstract

The invention relates to application of clomifene citrate to anti-mycobacterium tuberculosis medicines. Clomifene citrate has estrogenic and antiestrogenic properties that appear to prevent the release of gonadotropins, follicle-stimulating hormone and luteinizing hormone, thus leading to follicular development and maturation, ovulation and subsequent corpus luteum development and function and then resulting in pregnancy. It is found through researches that the clomifene citrate has an antitubercular effect and has good development value. The application of the clomifene citrate to anti-mycobacterium tuberculosis medicines and the obvious anti-mycobacterium tuberculosis effect of the clomifene citrate are disclosed for the first time.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to the application of clomiphene citrate in anti-mycobacterium tuberculosis drugs. Background technique [0002] Due to the lack of effective preventive vaccines, lack of effective diagnostic techniques, and the occurrence of drug-resistant and refractory tuberculosis, the prevention and control of tuberculosis is still an important scientific problem at present, and the first-line anti-tuberculosis drugs used today are all used more than 40 years ago. Research and development, the development of new and effective anti-tuberculosis drugs is slow, and these drugs directly act on the tuberculosis itself, and there is always a risk of cross-drug resistance. Host anti-tuberculosis immunity and inflammatory response are closely related to the outcome of tuberculosis infection. Therefore, by interfering with host functions to increase host anti-TB immunity, the purpose of eradicat...

Claims

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Application Information

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IPC IPC(8): A61K31/138A61P31/06
CPCA61K31/138A61P31/06
Inventor 蔡毅陈心春欧阳琪
Owner SHENZHEN UNIV
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