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Application of magnolin in resisting colorectal cancer

A technology of magnolanin and colorectal cancer, applied in the direction of antineoplastic drugs, drug combinations, active ingredients of heterocyclic compounds, etc., can solve the problem of low 5-year survival rate of colorectal cancer patients

Inactive Publication Date: 2019-10-22
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Colorectal cancer is one of the common malignant tumors. Although the mortality rate of colorectal cancer patients has been reduced by local intervention, early diagnosis and screening, surgery and other technical progress in the past decade, some colorectal cancer patients are still diagnosed. Distant metastases have been found at the time, and the 5-year survival rate of these patients with metastatic advanced colorectal cancer is still very low

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  • Application of magnolin in resisting colorectal cancer
  • Application of magnolin in resisting colorectal cancer
  • Application of magnolin in resisting colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, magnolanin anti-human colorectal cancer activity

[0050] 1. Magnolin inhibits the growth of human colorectal cancer cells

[0051] Cell viability was determined by MTT assay, and the growth inhibitory effect of magnolanin on two typical human colorectal cancer cell lines (human colorectal cancer cell line HCT116 and human colorectal adenocarcinoma cell line SW480) was analyzed. HCT116 and SW480 were cultured for 48 hours in culture media containing 0, 10 μM, 20 μM, 30 μM, and 40 μM magnolanin, respectively, and the cell viability was measured by MTT method. The results showed that 10 μM, 20 μM, 30 μM, and 40 μM magnolanin were treated for 48 hours , human colorectal cancer cell line HCT116 and human colorectal adenocarcinoma cell line SW480 colorectal cancer cell viability decreased significantly ( figure 2 ). It shows that magnolanin significantly inhibits the growth of human colorectal cancer cells and human colorectal adenocarcinoma cells.

[0052]...

Embodiment 2

[0066] Example 2, Magnolin promotes the autophagy of human colorectal cancer cells and human colorectal cancer

[0067]In addition to apoptosis, autophagy is another mode of programmed cell death in response to cellular stress. The effect of magnolanin on autophagosome formation was first investigated by evaluating LC-3B and p62, two classic autophagy markers, using media containing 0, 10, 20, 30, and 40 μM magnolanin, respectively. HCT116 and SW480 were cultured for 48 hours, and the cells were taken to analyze p62, LC-3B and Actin ( As an internal reference) expression, the results showed that magnolanin could up-regulate the expression of LC-3B in a certain dose-dependent manner, while the expression of p62 was significantly reduced ( Figure 13 Middle a). To further verify magnolanin-induced autophagy, intracellular morphological changes in colorectal cancer were investigated by transmission electron microscopy. The method is as follows: HCT116 and SW480 were respective...

Embodiment 3

[0070] Example 3, magnolanin inhibits LIF and MCL-1, promotes autophagy and inhibits cell cycle

[0071] To study the effect of magnolanin on signaling pathways, culture HCT116 and SW480 for 48 hours with culture medium containing 0, 10, 20, 30 and 40 μM magnolanin, respectively, and use Mcl-1 antibody, LIF antibody, anti-β -Actin (A5441) antibody was used to analyze the contents of Mcl-1, LIF and Actin (as an internal reference) by Western blot, and the results showed that magnolanin could inhibit the protein expression of Mcl-1 and LIF ( Figure 14 middle a, b).

[0072] For HCT116 and SW480, both were transferred into human Mcl-1 expression plasmid to overexpress Mcl-1 to obtain recombinant HCT116 / Mcl-1 and recombinant SW480 / Mcl-1 respectively. HCT116, recombinant HCT116 / Mcl-1, SW480 and recombinant SW480 / Mcl-1 were cultured for 48 hours with medium containing 0 μM and 20 μM magnolanin respectively, and the cells were taken and treated with anti-Mcl-1 antibody, anti-LC-3B ...

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Abstract

The invention discloses an application of magnolin in resisting colorectal cancer. Magnolin significantly inhibits the growth and proliferation of human colorectal cancer cells and human colorectal adenocarcinoma cells, significantly inhibits the growth of human colorectal cancer, and significantly inhibits the cell cycle of human colorectal cancer; magnolin promotes the autophagy of human colorectal cancer cells and human colorectal cancer. By blocking LIF / Stat3 / Mcl-1 pathways, magnolin can promote the autophagy of the colorectal cancer cells and colorectal cancer, inhibits the proliferationof the colorectal cancer cells and blocks the period of the colorectal cancer cells. Magnolin has the activity of resisting human colorectal cancer cells and human colorectal cancer, and can be used for preparing products for resisting colorectal cancer or resisting the colorectal cancer cells.

Description

technical field [0001] The invention relates to the medical application of magnolanin in the field of medicine, in particular to the application of magnolanin in antitumor (such as colorectal cancer). Background technique [0002] Magnolin is a chemical substance, its CAS number is 31008-18-1, and its structural formula is as follows figure 1 , Magnolin has strong anti-allergic and anti-inflammatory effects (Li Xiaoli, Zhang Yongzhong. Experimental research on the anti-inflammatory and anti-allergic effects of magnolia. "Chinese Herbal Medicine", Volume 33, Issue 11, 2002). [0003] Colorectal cancer is one of the common malignant tumors. Although the mortality rate of colorectal cancer patients has been reduced by local intervention, early diagnosis and screening, surgery and other technical progress in the past decade, some colorectal cancer patients are still diagnosed. Distant metastases have been found at that time, and the 5-year survival rate of these patients with m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/34A61P35/00A61P1/00
CPCA61K31/34A61P1/00A61P35/00
Inventor 于海洋邱玉玲王涛高秀梅吴淞张祎韩立峰庞旭
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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