A fluorescent molecular probe for detecting formaldehyde and its preparation method, and a preparation method of its fluorescent test paper
A fluorescent molecular probe, formaldehyde technology, applied in fluorescence/phosphorescence, chemical instruments and methods, material analysis by optical means, etc., can solve the problem that requires tens of minutes or even hours, and trace formaldehyde cannot be quantitatively detected. , unable to adapt to the complex and changeable environment and other problems, to achieve the effect of short response time, conducive to large-scale production, and reduce fluorescence loss
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Embodiment 1
[0036] R in the fluorescent probe structural formula prepared in this embodiment 1 = H, R 2 =H.
[0037] The synthesis technical route of the present embodiment is as follows:
[0038]
[0039] Synthesis of compound 2
[0040] First, weigh isatoic anhydride (1.0g, 6.13mmol) into a 350mL round bottom flask, add 15mL of glacial acetic acid, stir and mix at room temperature, then weigh 2-aminothiophenol (0.85mL, 7.97mmol) and add to the reaction system, and then the reaction system was moved to a water bath environment, the temperature of the water bath was set at 90°C, and the reaction time was 2h. During the reaction process, the reaction system gradually changed from a turbid state to a clear state, and the color gradually changed from taupe to orange-yellow; TLC monitored the reaction process, and the dark spots of raw materials gradually decreased, producing blue fluorescent spots with less polarity, and by-products less. After the reaction was finished, the reaction...
Embodiment 2
[0051] Preparation of probe master solution
[0052] Accurately weigh 4.3 mg of the fluorescent molecular probe prepared in Example 1, place it in a 50 mL brown volumetric flask, add DMSO:H at room temperature 2 O=1:1 to make it fully dissolved, and constant volume to the mark, the obtained solution is 1mM probe mother solution.
[0053] Preparation of formaldehyde mother liquor:
[0054] Use a pipette gun to accurately measure 76uL and 760uL of 37% formaldehyde aqueous solution; transfer them to 10mL volumetric flasks, add deionized water at room temperature to fully dissolve, and dilute to the mark to obtain a solution of 100mM and 1000mM formaldehyde stock solution.
[0055] Fluorescence Response of Fluorescent Probes to Formaldehyde
[0056] Take 20 μL of the probe mother solution and 0, 6, 10, 20, 30, 40, 60, 100, 200, 240, 300, 360, 400 μL of the formaldehyde mother solution and place them in the test system, fully dissolve, and the total volume of the test system fo...
Embodiment 3
[0059] The solution prepared in Example 2 was used as a fluorescent probe, and the selectivity of the probe to formaldehyde was evaluated. The excitation wavelength of the nanoprobe was 365 nm. We have selected a variety of common small molecule aldehyde-containing compounds: 1. Benzaldehyde, 2. Acetaldehyde, 3. Propionaldehyde, 4. Methyl formate, 5. Ethyl formate, 6. Glyoxal, 7. Acetone, 8. Acetic acid, 9. Furfural, and 10. Formaldehyde were formulated into aqueous solutions and added to the probe solution at a concentration of 20 μM, and the probe concentration was 10 μM, where DMF: H 2 O=1:1 (v / v), pH value is 7.0, measure its fluorescence emission spectrum and record the intensity of fluorescence emission at 525nm. The result is as image 3 As shown, it is found that the fluorescent probe only has obvious fluorescence enhancement at 525nm for formaldehyde, and the fluorescence changes for other tested small molecules are weak, indicating that it has excellent selectivity ...
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