Spectrum-based cell culture suspension pH measuring method

A technology of cell culture and detection methods, which is applied in the direction of color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions. Problems such as the pH value of the cell culture suspension, to achieve high-precision measurement and eliminate interference

Active Publication Date: 2019-10-25
世翱(上海)生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] At present, there is no relevant report on the use of s

Method used

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  • Spectrum-based cell culture suspension pH measuring method
  • Spectrum-based cell culture suspension pH measuring method
  • Spectrum-based cell culture suspension pH measuring method

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Embodiment 1

[0035] Such as figure 1 As shown, a method for detecting the pH value of a cell culture suspension based on a spectrum of the present invention includes the following steps:

[0036] 1. Transmission spectrum collection: Add acid-base indicator to cell culture suspension. Put the cell culture suspension containing the acid-base indicator (ie the suspension to be measured) into an optical container with a fixed optical path, use a light source to irradiate the cell suspension, and use a spectrometer to receive and measure the transmission spectrum.

[0037] 2. Spectrum value correction: Consider the influence of cell density on the transmission spectrum, and correct the transmission spectrum.

[0038] The correction formula is:

[0039]

[0040] In the formula, λ is the effective spectral wavelength; τ(λ) is the transmitted light intensity at the wavelength λ before conversion; Is the transmitted light intensity at the wavelength λ after conversion; lg is the logarithm of the base 10; ...

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Abstract

The invention discloses a spectrum-based cell culture suspension pH measuring method. An acid-base indicator which can change color with a pH value of a suspension is added in a cell culture suspension, then color of the cell suspension is measured by using transmitted spectrum, and the pH value of the suspension is determined. According to the method, the problem that an electrode type pH meter is not suitable for the pH value detection of the cell culture suspension is solved, the interference of the cell density on a pH measurement result also can be effectively eliminated, and the high-precision measurement of the pH value of the cell suspension is achieved.

Description

Technical field [0001] The invention belongs to the field of biological information detection, and specifically relates to a pH value detection method, in particular to a spectrum-based pH value detection method of cell culture suspension. Background technique [0002] The pH value is an important environmental detection index for the culture suspension during the suspension cell culture process. A pH meter refers to an instrument used to determine the pH value of a solution. The pH meter in industrial production uses the principle of the primary battery to work. According to Nerns's law, there is a corresponding relationship between the electromotive force between the two electrodes of the galvanic cell and the hydrogen ion concentration in the solution, and the negative logarithm of the hydrogen ion concentration is the pH value of the solution. However, before using the electrode type pH meter for measurement, it is necessary to rinse the electrode with distilled water and w...

Claims

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Application Information

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IPC IPC(8): G01N21/80G01N21/25
CPCG01N21/25G01N21/80
Inventor 冯伟兴顾莉萍
Owner 世翱(上海)生物医药科技有限公司
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