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Tissue culture technology breeding method of ainsliaea fragrans Champ

A technology of apricot fragrant rabbit ears and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of reproduction and cutting of wild resource offspring, reduction of wild resources reserves, miscellaneous varieties of apricot fragrant rabbit ears, etc. To achieve the effect of fast reproduction, high reproduction rate and maintaining parental traits

Inactive Publication Date: 2019-11-01
云南云龙制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sources of this plant as medicinal materials are all wild resources, and the whole plant is collected. The propagation method of wild resources is mainly seed propagation. The seed maturity time is generally from December to January of the next year, and the harvest time of medicinal materials is generally in the early stage of reproductive growth. (August-October), resulting in the cutting off of the offspring of wild resources, resulting in a decrease in the reserves of wild resources year by year
In recent years, some enterprises and institutions have carried out research on planting technology of apricot-fragrant rabbit's ears, but due to the time conflict between seed collection and medicinal material harvesting, the cultivation of apricot-fragrant rabbit's ears is in the early stage of development and has not yet formed a scale
At the same time, although Xingxiang rabbit's ear wind is widely distributed, there is a big difference in the content of active ingredients in the medicinal materials produced in different regions, and there are large differences in the quality of different batches or even the same batch of medicinal materials. The reason is that the variety of Xingxiang rabbit's ear wind Complicated, need to breed specific high-quality varieties for the production of medicinal materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Collect the wild plants of wild apricot and rabbit ears in April, transplant them in the laboratory, and after turning green in about 10 days, spray 75% carbendazim 500 times solution at about ten o'clock in the morning, spray once every three days, About 30 days after transplanting, take the young stems and cut them into 2~3cm size;

[0019] 2. Rinse the explants with a saturated soap solution for 10 minutes, and then rinse with water 4 times until no soap water remains, add 2 drops of Tween-80, shake for 15 minutes, rinse with tap water for 1 hour, pour out the tap water, and transfer to a clean bench Inside, disinfect with 75% alcohol for 25 seconds, rinse with sterile water for 3 times, then disinfect with saturated bleach solution for 20 minutes, rinse with sterile water for 3 times, and finally disinfect with 0.1% mercury liter solution for 7 minutes, rinse with sterile water for 6 times ;

[0020] 3. Use sterile filter paper to dry the surface of the steri...

Embodiment 2

[0024] 1. Collect the wild plants of wild apricot and rabbit ears in June, transplant them in the laboratory, and after turning green in about 10 days, spray 75% carbendazim 500 times solution at about 10:00 in the morning, and spray once every 4 days. About 50 days after transplanting, take the young leaves and cut them into 3~4cm2 size;

[0025] 2. Rinse the material with saturated soapy water solution for 15 minutes, then rinse with clean water 3 times until no soapy water remains, add 3 drops of Tween-80, shake for 20 minutes, rinse with tap water for 80 minutes, pour out the tap water, and transfer it to an ultra-clean workbench. Disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 3 times, then disinfect with saturated bleach solution for 15 minutes, rinse with sterile water for 3 times, and finally disinfect with 0.1% mercuric solution + 2 drops of Tween-80 for 8 minutes. Rinse with water 8 times;

[0026] 3. Use sterile filter paper to dry the su...

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Abstract

The invention discloses a tissue culture technology breeding method of ainsliaea fragrans Champ. The method comprises the steps of selection of explants, cleaning and disinfection of the explants, induction of cluster buds, multiplication and rooting of the cluster buds and the like. According to the method, a tissue culture technology of ainsliaea fragrans Champ is explored, ideal culture conditions such as acquisition of an aseptic material, induction of calluses, differentiation of the calluses into buds and rooting are determined, finally, an asexual reproduction system of tissue culture seedlings of ainsliaea fragrans Champ is established, a technical foundation is laid for protecting and cultivating wild resources of ainsliaea fragrans Champ, meeting demands for seedlings and establishing a gene bank, and the problems of low yield, small supply amount and long natural cultivation cycle of wild panax japonicus majoris are solved.

Description

technical field [0001] The invention relates to a tissue culture method of Chinese medicinal materials, which belongs to the field of plant tissue culture and planting of Chinese medicinal materials, in particular to a tissue culture method of apricot-flavored rabbit's ear wind. Background technique [0002] Ainsliaea fragrans Champ is a plant of the Asteraceae Ainsliaea fragrans Champ. The whole herb is used as medicine. It has the effects of clearing away heat and detoxification, promoting dampness, and stopping bleeding. It is mainly used to treat lung abscess, mastitis, malnutrition, osteomyelitis, and snake bite hurt. Apricot rabbit ear wind is mainly distributed in Taiwan, Fujian, Zhejiang, Anhui, Jiangsu, Jiangxi, Hubei, Sichuan, Hunan, Guangdong, Guangxi, Yunnan and other provinces. In the border grass, Yunnan is also distributed near the altitude of 2000 meters. At present, the sources of this plant as medicinal materials are all wild resources, and the whole plan...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨建云邓清曾祥飞曾琼赵露琴
Owner 云南云龙制药股份有限公司
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