Method for quick breeding of mesembryanthemum crystallium tissue

A fast and ice-cooked technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to improve the survival rate, reduce the pollution rate, and promote the effect of factory seedling raising

Inactive Publication Date: 2019-11-12
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no method for mesophyll tissue

Method used

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  • Method for quick breeding of mesembryanthemum crystallium tissue
  • Method for quick breeding of mesembryanthemum crystallium tissue
  • Method for quick breeding of mesembryanthemum crystallium tissue

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The present embodiment carries out rapid tissue breeding with Shandong Shouguang (ice vegetable variety) as an example, specifically comprising the following steps:

[0029] 1) The cultivation of sterile seedlings comprises the following steps: a. prepare 1 / 2 of the MS medium, add 7.5g agar (mass fraction is 32%), after all dissolving, medium and related instruments are treated by high temperature sterilization Use; b. Put the seeds into the vacuum pump and soak them in 10% sodium hypochlorite solution for 3 times, each time for 10-15s, and then rinse with sterile water several times; c. Put the seeds on the culture medium in the ultra-clean workbench, seal to cultivate. Culture conditions: temperature (23±2)°C, light intensity 1500lux, light 14h / d per day; when 3 true leaves grow and the length of the leaves is 5-6cm, sterile seedlings are obtained.

[0030] 2) Callus culture: callus medium includes: MS+6-BA 1.0 mg / L+NAA 0.15 mg / L. On the ultra-clean workbench, quick...

Embodiment 2

[0038] The present embodiment carries out rapid tissue breeding with Shandong Shouguang (ice vegetable variety) as an example, specifically comprising the following steps:

[0039] 1) the cultivation of sterile vaccine, step is with embodiment 1.

[0040] 2) Callus culture: callus medium includes: MS+6-BA 2.0 mg / L+NAA 0.5 mg / L. On the ultra-clean workbench, quickly cut the 1-2cm of mesophyll exposed by the palisade tissue and spongy tissue without veins and stems from the sterile seedlings, and inoculate them on the callus medium for callus induction, each bottle Inoculate 3 pieces of callus, a total of 60 bottles; culture conditions: dark treatment for 48h after inoculation; temperature (25±2)°C, light intensity 150lux, light time 24h / d, culture for 45 days, and callus formation. The callus browning rate was 3%, the contamination rate was 58.8%, and the survival rate was 27.5%.

[0041] 3) Adventitious bud differentiation: Adventitious bud differentiation medium includes: M...

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Abstract

The invention relates to a method for quick breeding of mesembryanthemum crystallium tissue and belongs to the technical field of mesembryanthemum crystallium cultivation. The method includes: sterileseedling culture, callus culture, adventitious bud differentiation, transgenerational propagation, rooting and seedling strengthening, and transplanting. Mesophyll tissue of sterile seedlings is utilized, a blade is utilized for cutting, and exposed palisade tissue and spongy tissue are made to contact with a culture medium as much as possible. The sterile seedlings are cultivated for propagation, so that contamination rate and browning rate of callus are lowered, and experimental survival rate is increased; by the method, a lot of mesembryanthemum crystallium seedlings can be obtained through tissue propagation, so that industrialized seedling culture of mesembryanthemum crystallium is promoted.

Description

technical field [0001] The invention belongs to the technical field of ice vegetable cultivation, and in particular relates to a method for rapidly growing ice vegetable tissue. Background technique [0002] Ice leaf Rizhonghua (scientific name: Mesembr yanthemum crystallinum Linn.) is an annual or biennial herb of the Apricot family and the genus Rizhonghua. It is a new type of health-care vegetable. Its leaves have ice crystals and are unique. No fibrous tissue, suitable for all ages. Iced vegetables have strong resistance, few pests and diseases, and high quality, which is a good choice for increasing farmers' income and increasing social efficiency. The economic benefits of ice vegetables are relatively high, but the introduction time in our country is short and the cultivation technology is immature. [0003] At present, the traditional propagation methods of ice vegetable mainly include sowing, branching, grafting and layering, etc., but the seeds of ice vegetable ar...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 苏友波张永慧李贝贝林春徐权杰
Owner YUNNAN AGRICULTURAL UNIVERSITY
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