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25-hydroxyvitamin D content measurement method

A technology for the content of hydroxyvitamins, which is applied in the field of analysis and detection, can solve the problems of microsphere aggregation, uneven dispersion, reduction, etc., and achieve the effect of short time consumption and simple operation

Active Publication Date: 2019-11-12
BEIJING DIAGREAT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the fluorescent microspheres of the traditional fluorescent immunochromatography test strips are sprayed on the glass fiber, and the microspheres are prone to aggregation, uneven dispersion, poor release effect, and antibody activity reduction or destruction during the drying process, resulting in inaccurate test results. , large fluctuations or reduced sensitivity

Method used

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Embodiment 1

[0045] 1. Preparation of reagents:

[0046] Dissociation solution: the pH value is 7.4, the barbital sodium buffer solution that concentration is 200mmol / L is mixed with saponin, Tween-20, wherein, the content of saponin in the described dissociation solution is 2wt.%. The content of Wen-20 is 1wt.%;

[0047] Diluent: mix boric acid-borax buffer solution with a pH value of 8.0 and a concentration of 50mmol / L with bovine serum albumin, trehalose, polyvinylpyrrolidone, and preservative proclin 300, wherein the bovine serum albumin in the diluent The content of trehalose is 0.5wt.%, the content of trehalose is 1wt.%, the content of polyvinylpyrrolidone is 0.5wt.%, and the content of preservatives is 0.1wt.%.

[0048] Fluorescent microsphere working solution: mix anti-25-hydroxyvitamin D antibody-labeled fluorescent microspheres (labeled amount: 100 μg / mL), anti-chicken IgY-labeled fluorescent microspheres (labeled amount: 200 μg / mL) and diluent , controlling the dilution factor...

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Abstract

The invention relates to the technical field of analysis and detection, and in particular relates to a 25-hydroxyvitamin D content measurement method. According to the method, 25-hydroxyvitamin D is dissociated from vitamin D binding protein in a biological sample through a dissociation liquid; the dissociation liquid is mild in components and free of damage to an antibody; therefore the biological sample, the dissociation liquid and a fluorescent microsphere working solution can be jointly incubated, so that an anti-25-hydroxyvitamin D antibody in the fluorescent microsphere working solutionis fully combined with the 25-hydroxyvitamin D dissociated from the biological sample, the sensitivity can be effectively improved, and the linear range is 3-100 ng / mL.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for measuring the content of 25-hydroxyvitamin D. Background technique [0002] Vitamin D is a kind of fat-soluble vitamin, which belongs to sterol derivatives, and its molecule contains cyclopentane polyhydrophenanthrene structure. It comes in many forms, mainly including vitamin D 1 、D 2 、D 3 、D 4 and D 5 , the most important of which is vitamin D 2 (ergocalciferol, ergocalciferol) and D 3 (Cholecalciferol, cholecalciferol), commonly referred to as vitamin D refers to these two forms. The main physiological function of vitamin D is to promote the absorption of calcium and phosphorus and promote osteogenesis. The lack of vitamin D can easily lead to rickets and rickets, and excessive intake can cause potential toxicity. [0003] In the body, vitamin D needs to be hydroxylated by the liver and kidneys to form 25-hydroxyvitamin D and 1,25-dihydroxyv...

Claims

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Application Information

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IPC IPC(8): G01N33/82G01N21/64
CPCG01N33/82G01N21/6428G01N2021/6439
Inventor 周建平王艳敏
Owner BEIJING DIAGREAT BIOTECH CO LTD
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