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Molecular marker for kidney disease diagnosis and application thereof

A technology for kidney disease and function, applied in the medical field, can solve unclear problems and achieve the effect of simple method, great theoretical significance, and easy promotion

Pending Publication Date: 2019-11-19
NINGXIA MEDICAL UNIV
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Problems solved by technology

However, the molecular mechanism of HHcy-induced kidney injury is still unclear, so it is necessary to find potential early diagnosis and adopt more effective treatment methods

Method used

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  • Molecular marker for kidney disease diagnosis and application thereof
  • Molecular marker for kidney disease diagnosis and application thereof
  • Molecular marker for kidney disease diagnosis and application thereof

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Embodiment Construction

[0021] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0022] Concrete technical scheme of the present invention is:

[0023] (1) Establishment of HHcy animal model induced by high methionine diet: choose Cbs + / + and Cbs + / - Mice, two groups of mice were fed with 2% high methionine diet, after feeding for 8 weeks, follow-up experiments were carried out.

[0024] (2) Take blood from the eyeball, separate mouse serum, detect serum Hcy levels, creatinine (SCr), blood urea nitrogen (BUN), and cystatin C (Cyt C) levels with an automatic biochemical analyzer; observe glomeruli under a transmission electron microscope Ultrastructural changes; Glycogen staining observed renal histopathological changes.

[0025] (3) Detect the expression of miR-30a-5p in mouse kidney tissue, and construct a stable cell line with miR-30a-5p overexpression and interference, and observe its effect...

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Abstract

The invention discloses a molecular marker for kidney disease diagnosis and an application thereof, and belongs to the technical field of medicine, and relates to an application of a reagent in preparation of kidney disease diagnosis products, wherein the reagent is used for detecting the expression level of miR-30a-5p in an animal tissue sample. The miR-30a-5p has the sequence shown in SEQ:ID:No:1. The reagent detects the expression level of miR-30a-5p in the process of HHcy induced renal injury. An overexpressed and silence miR-30a-5p virus is synthesized in vivo and in vitro, an effect on renal injury is studied, and the role of miR-30a-5p in the process of HHcy induced renal injury is clarified. The miR-30a-5p plays an important role in the process of HHcy induced renal injury, provides a theoretical basis for later diagnosis of renal diseases in the molecular level, and has great theoretical significance and potential practical value. The method is simple and easy to do, and easyto promote.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to a molecular marker for kidney disease diagnosis and application thereof. Background technique [0002] Homocysteine ​​(Hcy) is a sulfur-containing amino acid and a metabolite of methionine in the body. The American Heart Association clearly defines hyperhomocysteinemia (HHcy) as total plasma Hcy levels higher than 10 μmol / L. With the development and deepening of research on Hcy, a large number of studies have shown that HHcy can not only be used as an independent risk factor for cardiovascular diseases such as atherosclerosis and hypertension, but also related to the occurrence and development of various kidney diseases, which is the cause of renal small Independent risk factors for renal function injury such as glomerular injury and decreased filtration rate. The glomerular filtration barrier is composed of the innermost layer of glomerular endothelium, the middle layer of glom...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 姜怡邓张慧萍谢琳丁宁刘昆马胜超王艳华卢冠军
Owner NINGXIA MEDICAL UNIV
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