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Extraction method of extracting cyclic peptide of heterophylla by using surfactant

The technology of a surfactant and an extraction method is applied in the extraction field of extracting the cyclopeptide by using the surfactant, which can solve the problems of high production cost, long labor time, environmental pollution of organic solvents, etc., and achieves low cost and simple equipment. , the effect of efficient extraction

Active Publication Date: 2021-06-15
NINGDE NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the extraction technology of Pseudostellaria cyclic peptide still stays in the traditional technology, such as conventional alcohol extraction, silica gel column chromatography, etc., not only the production cost is high, labor is time-consuming, but also a large amount of organic solvents are used to cause serious environmental pollution

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  • Extraction method of extracting cyclic peptide of heterophylla by using surfactant
  • Extraction method of extracting cyclic peptide of heterophylla by using surfactant

Examples

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Embodiment 1

[0034] Example 1 is when the surfactant used in step 3) is the anionic surfactant sodium dodecylbenzenesulfonate, the extraction of heterophyllin. The extraction method of the heterophyllin cyclic peptide is specifically:

[0035] Step 1) Pretreatment of Radix Pseudostellariae: Wash, freeze-dry and crush Pseudostellariae, pass through a No. 3 sieve, add distilled water according to the mass ratio of solid to liquid at 1:20g / mL, let stand and soak at room temperature for 4 hours, and obtain the distilled water soaked product of Pseudostellariae Radix.

[0036] Step 2) Separation of hot water: heat the stewed ginseng soaked in distilled water obtained in step 1) to 90°C, and continue to stir for 2.5 hours, then filter out the residue of heterophylla heterophylla to obtain a mixture of heterophylla pseudostellariae and hot water, which is passed through hot water Put the Pseudostellariae-hot water mixture obtained from preliminary separation into the continuous foam separation to...

Embodiment 2 Embodiment 6

[0040] Embodiment two to embodiment six are: the anionic surfactant sodium dodecylbenzenesulfonate used in step 3) in embodiment one is replaced by fatty alcohol ether sodium sulfate, fatty acid methyl ester ethoxylate sulfonic acid respectively Sodium, sodium secondary alkyl sulfonate, sodium alcohol ether carboxylate and sodium alcohol ether phosphate, the extraction of heterophyllin.

[0041] Wherein, the extraction rate of embodiment 1 to embodiment six Taizi ginseng peptide is shown in Table 1.

[0042] The extraction rate data of Taizishen cyclic peptide in the embodiment one to embodiment six of table 1

[0043] Example Anionic Surfactant Pseudostellaria cyclic peptide extraction rate Embodiment one Sodium dodecyl benzene sulfonate 91.3％ Embodiment two Sodium Fatty Alcohol Ether Sulfate 94.5％ Embodiment three Sodium fatty acid methyl ester ethoxylate sulfonate 91.6％ Embodiment four Sodium secondary alkyl sulfonate 92.2％ ...

Embodiment 7

[0045] Example 7 is when the surfactant used in step 3) is cationic surfactant fatty alcohol polyoxyethylene ether ammonium sulfate, the extraction of heterophyllin. The extraction method of the heterophyllin cyclic peptide is specifically:

[0046] Step 1) Pseudostellaria pretreatment: Wash, freeze-dry and crush Pseudostellariae, pass through a No. 3 sieve, add distilled water according to the mass ratio of material to liquid at 1:15g / mL, let stand and soak at room temperature for 5 hours, and obtain the distilled water soaked product of Pseudostellariae.

[0047] Step 2) Separation of hot water: heat the stewed ginseng soaked in distilled water obtained in step 1) to 85°C and continue stirring for 3 hours, then filter out the residue of heterophylla heterophylla to obtain a mixture of heterophylla pseudostellariae and hot water, which is preliminarily mixed with hot water Put the separated Pseudostellariae-hot water mixture into the continuous foam separation tower, control ...

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Abstract

The present invention relates to a kind of extraction method that utilizes surfactant to extract Radix Pseudostellariae cyclic peptide, and it comprises the following steps: 1) Pseudostellariae pretreatment; The mixed solution is put into the continuous foam separation tower; step 3) surfactant extraction: in the continuous foam separation tower equipped with heterophyllaphylloides-hot water mixed solution, add surfactant to the Pseudostellaria cyclic peptide The hot water mixture is extracted to obtain a crude extract; step 4) purification of the crude extract: add a regulator that can provide a counterion corresponding to the surfactant in the obtained crude extract, filter to remove the precipitate, and collect the resulting filtrate That is, Pseudostellaria cyclic peptide extract. The present invention utilizes the surfactant and combines the foam separation technology to efficiently extract the heterophylla cyclic peptide, and the extraction rate of the heterophyll cyclic peptide can reach more than 90%, which shows that the method is an extremely effective extraction method.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of natural active ingredients, and in particular relates to an extraction method for extracting heterophyllin by using a surfactant. Background technique [0002] Pseudostellaria heterophylla (Miq.) Pax is the dried tuber root of Pseudostellaria heterophylla (Miq.) Pax, a commonly used traditional Chinese medicine. Modern medicine shows that the active ingredients in Radix Pseudostellariae are mainly trace elements, amino acids, sugars, glycosides, cyclic peptides, phospholipids, fatty acids, fats and volatile oils (such as figure 1 High-performance liquid phase (HPLC) fingerprints of organic matter in the shown Radix Pseudostellariae Pseudostellariae). Among them, as an important class of peptide compounds, Pseudostellaria cyclic peptides with biological activity show better resistance to enzymolysis and chemical degradation in vivo than the corresponding linear peptides, and are widely...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 梁志瑜黄仁昆颜桂炀高冰
Owner NINGDE NORMAL UNIV
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