Experimental method for simultaneous record and research of monophasic and biphasic action potentials of nerve trunk

Abstract

An experimental method for the simultaneous record and research of monophasic and biphasic action potentials (AP) of a nerve trunk comprises the following steps: (1) an isolated nerve trunk is prepared, an O-ring is formed in the middle of the nerve trunk, the cross node of a first half and a second half of the nerve trunk is bound with a thin line, and the O-ring is cut; (2) the nerve trunk is put into a multi-electrode neural specimen box, and the cross node of the nerve trunk is located between a second recording electrode and a third recording electrode; (3) a first stimulating electrode and a second stimulating electrode are powered on, the biphasic AP between a first recording electrode and the second recording electrode is recorded, and the monophasic AP between the first recordingelectrode and the third recording electrode is recorded; and (4) the biphasic AP and the monophasic AP obtained in step (3) are analyzed and compared. The experimental method of the invention can be used to accurately compare and analyze the similarities, differences and internal relations of the biphasic and monophasic APs at the same time between the same two points of a nerve trunk under the same stimulation condition.

Description

technical field [0001] The invention belongs to the technical field of physiological experiment research, and in particular relates to an experimental method for simultaneous recording and research of single-biphasic action potential of a nerve trunk. Background technique [0002] Nerve trunk (nerve trunk) is covered by epineurium and contains many nerve fibers. Nerve fibers realize rapid long-distance transmission of information by generating and conducting action potentials. Nerve trunk action potentials (action potentials, AP) are the comprehensive electrical signals (compound action potentials) that are generated by multiple nerve fibers in the nerve trunk and transmitted to the surface of the nerve trunk to be recorded. The classic content of learning experiment teaching and research. [0003] According to different recording conditions, neural trunk action potential can be divided into neural trunk biphasic action potential and neural trunk monophasic action potentia...

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Problems solved by technology

[0006] Similarly, since existing experimental methods cannot accurately compare and analyze the characteristics of biphasic AP and monophasic AP between the same two points under the same stimulation conditions, the neural trunk biphasic AP given in existing related books The peak value of the waveform and the single-phase AP waveform are all of the same height. For related content, please refer to "Analysis of Physiological Hundred Words", edited by Cui Gengyin, presided over by Gu Shuangzhen and Guan Zhenlong, China Press of Traditional Chinese Medicine, Beijing, 2005, P34-36; "Animal Physiology", 3rd edition, edited by Chen Shouliang, 2005, P29-31, Peking University Press; "Experimental Physiology", edited by Wang Tinghuai, Yang Huiling, Wang Xuelan, 2014, P138-139, Higher Education Press; Experimental Science (Bilingual Textbook), edited by Xie Keming, Wang Guoqing, Jiang Xinghong, Sheng Rui, etc., 2014, P113-114, Higher Education Press; "Experimental Course of Medical Functional Science", 4th edition, edited by Hu Huanzhong, Mou Yangling, etc. , 2016, P101, Science Press; etc.

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