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Cell culture method and cell culture device capable of generating variable pulsed electric field

A pulsed electric field and cell culture technology, applied in the field of cell culture, can solve problems such as limited knowledge and unclear differences in the treatment effect of optimal treatment conditions.

Active Publication Date: 2019-11-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current research on the mechanism of irreversible electroporation on cells is still relatively preliminary, and the relevant knowledge is still very limited. The reasons for the different outcomes of cells after electric field treatment, the optimal treatment conditions of electric field, and the treatment that may be caused by in vivo and in vitro experiments The difference in effect, etc. is not yet clear

Method used

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  • Cell culture method and cell culture device capable of generating variable pulsed electric field
  • Cell culture method and cell culture device capable of generating variable pulsed electric field
  • Cell culture method and cell culture device capable of generating variable pulsed electric field

Examples

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Effect test

Embodiment 1

[0061] A cell culture device capable of generating a variable pulse electric field. The device is a box with heating tubes 18 and O on the side wall of the box. 3 The through hole 25 is provided with a cooling tube 19, CO 2 Access hole 22, the back wall of the box is equipped with a temperature sensor 12, CO 2 Sensor 13 and O 3 Sensor 14. An electrode device 11, a stepping motor 26, and an LED lamp 27 are mounted on the top of the box. The electrode device 11 is an array electrode with a plurality of electrode needles. A stage 28 that can fix the culture dishes is provided in the device, and the stage 28 is equipped with a position sensor 15, CO 2 Generating device 20, O 3 The generating device 23 passes through the pipeline and CO 2 Access hole 22, O 3 The inlet holes 25 respectively pass CO into the culture device 2 , O 3 , The upper computer 2 is respectively connected to the temperature sensor 12, CO via the main control module 1 2 Sensor 13, O 3 Sensor 14, position sensor 15...

Embodiment 2

[0073] A cell culture device capable of generating a variable pulse electric field. The device is a box with heating tubes 18 and O on the side wall of the box. 3 The through hole 25 is provided with a cooling tube 19, CO 2 Access hole 22, the back wall of the box is equipped with a temperature sensor 12, CO 2 Sensor 13 and O 3 Sensor 14. An electrode device 11, a stepping motor 26, and an LED lamp 27 are mounted on the top of the box. The electrode device 11 is an array electrode with a plurality of electrode needles. A stage 28 that can fix the culture dishes is provided in the device, and the stage 28 is equipped with a position sensor 15, CO 2 Generating device 20, O 3 The generating device 23 passes through the pipeline and CO 2 Access hole 22, O 3 The inlet holes 25 respectively pass CO into the culture device 2 , O 3 , The upper computer 2 is respectively connected to the temperature sensor 12, CO via the main control module 1 2 Sensor 13, O 3 Sensor 14, position sensor 15...

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Abstract

The invention relates to a cell culture method and a cell culture device capable of generating a variable pulsed electric field. The cell culture device comprises a box body, a heating pipe and an O3inlet hole are arranged on the side wall of one side of the box body, a cooling pipe and a CO2 inlet hole are arranged on the other side wall of the box body corresponding to the heating pipe, a temperature sensor, a CO2 sensor and an O3 sensor are arranged on the rear wall of the box body, and an electrode device, a stepping motor and an LED lamp for illumination are arranged at the top of the box body; an objective table capable of fixing a culture dish is arranged below the electrode device in the box body; a position sensor is arranged on the objective table and is used for detecting a distance between the electrode device and the objective table; a setting control system comprises an upper computer which is used for setting parameters of a main control module, and the main control module is used for controlling pulse, temperature, CO2 concentration, O3 concentration and the distance between the electrode device and the objective table. The electric field intensity, the electric field frequency, the electric field polarity and the action time of the pulsed electric field are adjustable, and the limitation that a traditional device can only conduct external cell treatment is overcome.

Description

Technical field [0001] The field of cell culture of the present invention is specifically a cell culture method and a cell culture device capable of generating a variable pulse electric field. Background technique [0002] Cancer is the main disease endangering human health. Traditional tumor therapy and the newly developed thermal ablation physical therapy characterized by minimally invasive ablation are limited by indications, contraindications, treatment side effects, thermal effects and other factors, making their clinical applications have certain limitations. [0003] In recent years, with the continuous research and development of the mechanism of pulsed bioelectricity and related technologies, the pulsed electric field has attracted wide attention from researchers all over the world with its unique non-thermal biomedical effects, and has gradually been applied to the treatment of tumors. It has also achieved satisfactory therapeutic effects in clinical applications. [0004...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/00
CPCC12M41/12C12M41/34C12M31/10C12M21/02C12M41/48C12M37/00
Inventor 林婷婷王倩徐奡澍胡智能
Owner JILIN UNIV