Mycorrhizal Seedling Cultivation Method of Live Mycelia Net Infection Plant

Abstract

The invention discloses a mycorrhizal seedling raising method for living mycelium nets to infect plants. The aseptic seedling root system of the plant is penetrated from the outside of the culture container for upside-down planting, and the mycorrhizal fungus agent is inoculated around the root system; the upper layer of the isolation net The frame is closely attached to the inner wall of the culture container; the upper substrate is filled on the isolation net; the nutrient solution is poured and the excess water is drained through the drainage holes; after being inoculated with the bacterial agent and cultivated for six weeks, the plant root system can be mixed with the bacterial agent The mycorrhizal fungi established a symbiotic relationship. After the seeds sown on the upper layer germinated, the plants planted upside down at the bottom were shaded. After three weeks, the seedlings on the upper layer could be successfully mycorrhized. The invention is applicable to various mycorrhizal fungi, and can carry out mycorrhizal seedling cultivation on the upper layer of the culture container for many times, and the cycle is short, the operation is simple, and there is no need for multiple cultures of mycorrhizal fungal agents and multiple inoculations, and the bottom plants can be planted upside down once And inoculation, mycorrhizal seedlings can be carried out many times throughout the year. It is easy to use, saves time and effort, and has high feasibility.

Description

technical field [0001] The invention relates to a method for growing seedlings by mycorrhizalization, in particular to a method for growing seedlings by mycorrhizalization of living mycelia nets infecting plants, and belongs to the field of microorganisms. Background technique [0002] Mycorrhizal fungi are a class of fungi that are widely symbiotic with plants in the soil. There are various types of fungi, which can be briefly summarized as ectomycorrhizal fungi, arbuscular mycorrhizal fungi and orchid mycorrhizal fungi. Since mycorrhizal fungi can establish The relationship of mutual benefit and symbiosis, people widely put it into practical production application and study its symbiotic mechanism. Whether it is the actual production application of mycorrhizal fungi or the study of the symbiotic mechanism, it is inevitable to establish a symbiotic relationship between mycorrhizal fungi and plants, that is, to make the plant root mycorrhizal. [0003] The traditional mycor...

AI Technical Summary

Problems solved by technology

Different mycorrhizal fungi have different types of inoculum. Arbuscular mycorrhizal fungi generally use spores as inoculum for inoculation, and ectomycorrhizal fungi and orchid mycorrhizal fungi generally use solid cakes or liquid mycelium balls of fungi as inoculum. Inoculation, and the preparation methods of the above three mycorrhizal fungal agents are different. For the traditional mycorrhizal method, not only the preparation methods of mycorrhizal fungal agents are different, but also the recovery of mycorrhizal fungi and spore germination also require a lot of time , and every time mycorrhizal seedlings need to prepare mycorrhizal fungal agent, so the traditional mycorrhizal method is time-consuming and laborious

At present, there is no simple and practical mycorrhizal seedling cultivation method that is easy to mycorrhize seedlings and does not need to prepare mycorrhizal fungal agents multiple times, and is suitable for various mycorrhizal fungi.

Images