Trachemys scripta elegans detection method and PCR primer for detecting trachemys scripta elegans
A detection method, the technology of red-eared turtles, which is applied in the field of PCR primers for detection of red-eared turtles, can solve the problems of time-consuming and labor-intensive identification and low efficiency of identifying and monitoring red-eared turtles, and achieve high efficiency and reduce time-consuming and cost-effective effects
Active Publication Date: 2019-12-13
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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[0004] However, the above-mentioned existing prevention and control methods for red-eared turtles are time-consuming and labor-intensive, and the efficiency of identifying and monitoring red-eared turtles is relatively low
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[0035] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. The components of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations.
[0036] It should be noted that like numerals and letters denote similar items in the following figures, therefore, once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.
[0037] An embodiment of the present invention provides a method for detecting red-eared turtles, which can be applied to the investigation an...
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The invention provides a trachemys scripta elegans detection method and a PCR primer for detecting trachemys scripta elegans and relates to the technical field of trachemys scripta elegans detection.Multiple groups of DNA corresponding to different kinds of turtles can be acquired, a forward primer KM216748F and a reverse primer KM216748R are adopted, wherein a primer sequence of the forward primer KM216748F is 5'-TCTCAATTTTTCTTCTAGGGT-3', a primer sequence of the reverse primer KM216748R is 5'-GTTAGATGTTGTGGGTTA-3', multiple groups of DNA are subjected to PCR amplification reaction to obtainmultiple groups of corresponding PCR primers, the multiple groups of PCR products are subjected to electrophoresis detection separately, DNA corresponding to PCR products with electrophoresis bands in electrophoresis results is determined as DNA of the trachemys scripta elegans, specific detection of the trachemys scripta elegans is realized, effective means can be provided for rapid recognitionof field distribution of the alien invasive species trachemys scripta elegans, time and cost for control of the trachemys scripta elegans can be reduced, and the recognition and monitoring efficiencyof trachemys scripta elegans are high.
Description
technical field [0001] The invention relates to the technical field of red-eared turtle detection, in particular to a method for detecting red-eared turtles and PCR primers for detecting red-eared turtles. Background technique [0002] The red-eared turtle (Trachemys scripta elegans) is native to the central part of the United States and has been introduced to many countries in Europe, Africa, Asia, and the Americas. It poses a serious threat to the ecological security of the invaded area and is listed as a world species by the International Union for Conservation of Nature (IUCN). One of the top 100 most threatened invasive species. The red-eared turtle was introduced into the mainland of my country via Hong Kong in the 1980s. At the beginning of the 21st century, artificial breeding has formed a large scale. At the same time, due to artificial release and other reasons, it has entered the wild natural environment and is spreading. It has seriously threatened the habitat of...
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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2565/125Y02A90/40
Inventor 董姗姗章嫡妮刘燕张振华卢晓强于赐刚
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA