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Early liver cancer rat model construction and evaluation method

A rat model and early liver cancer technology, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve the problems of low sensitivity, time-consuming and labor-intensive, poor accuracy, etc., and achieve a strong specific effect

Active Publication Date: 2019-12-13
SHANXI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the construction and evaluation of early liver cancer rat models to solve the problems of poor accuracy, low sensitivity and time-consuming and laborious problems in the current construction and evaluation methods of early liver cancer rat models

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  • Early liver cancer rat model construction and evaluation method

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Embodiment 1

[0045] A method for constructing an early-stage liver cancer rat model, comprising the following steps:

[0046] Rats weighing 180-220 g were allowed to freely drink diethylnitrosamine solution with a concentration of 60 mg / L (prepared by sterilized water), which was changed every day according to the drinking conditions.

[0047] An evaluation method for early-stage liver cancer rat model, which is evaluated by the changes of endogenous metabolites in the body's end product urine before and after modeling, and the metabolic profile is characterized by using multivariate statistical analysis method. Perform pattern recognition on the data to examine the separation of the contours of each group of data, specifically:

[0048] At the 8th, 10th, and 12th weeks after the rat model was constructed, the urine of the rats in the blank and model groups were collected respectively. The urine was analyzed by nuclear magnetic resonance, and the 1H-NMR spectrum of the rat model was obtai...

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Abstract

The invention discloses an early liver cancer rat model construction and evaluation method, and belongs to the technical field of model construction and evaluation methods. The scheme of the inventionmainly solves the problems of poor accuracy, low sensitivity, time waste and energy waste of a current early liver cancer rat model construction and evaluation method. The scheme of the invention adopts a metabonomics technology, and analyzes changes in endogenous metabolites in urine, the end product of the body before and after modeling, to obtain a dynamic trajectory map of the metabolic profile of the pathological process. In addition, all nuclear magnetic resonance spectrums are processed by using MestReNova software to obtain integral data, the content of 19 biomarkers is combined for statistical analysis, and results show that the change of the integral mean value of the 19 biomarkers in the urine before and after modeling reflects the change trend of the urine metabolic process ofrats with liver cancer. The method of the invention provides a detection method that is simple to obtain specimens, and is more economical, safe and effective, and provides a high-sensitivity, high-throughput, non-invasive, and highly-specific evaluation method for new drug research and development and pharmacological research.

Description

technical field [0001] The invention belongs to the technical field of model construction and evaluation methods, and in particular relates to a construction and evaluation method of an early stage liver cancer rat model. Background technique [0002] Liver cancer is one of the malignant tumors with the highest mortality in the world, and its mortality rate has been increasing year by year in the past 20 years. my country is one of the countries with a high incidence of liver cancer, and its incidence ranks second in the incidence of malignant tumors in my country. Liver cancer is a canceration caused by the uncontrolled growth of liver cells caused by many factors such as viruses and chemical carcinogens. However, the exact etiology and pathogenesis of liver cancer are not fully understood. [0003] At present, the determination of the success of the replication of the rat primary liver cancer model is mainly based on histopathological examination, detection of serum alph...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N24/08
CPCG01N24/087G01N33/5091
Inventor 高丽王珂欣杜冠华秦雪梅
Owner SHANXI UNIV
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