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Construction and evaluation method of a rat model of early liver cancer

A rat model, the technology of early stage liver cancer, applied in the direction of instruments, analysis and measurement devices using nuclear magnetic resonance, can solve the problems of low sensitivity, poor accuracy, time-consuming and labor-intensive, and achieve the effect of strong specificity

Active Publication Date: 2021-03-30
SHANXI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the construction and evaluation of early liver cancer rat models to solve the problems of poor accuracy, low sensitivity and time-consuming and laborious problems in the current construction and evaluation methods of early liver cancer rat models

Method used

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  • Construction and evaluation method of a rat model of early liver cancer
  • Construction and evaluation method of a rat model of early liver cancer
  • Construction and evaluation method of a rat model of early liver cancer

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Experimental program
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Effect test

Embodiment 1

[0045] A method for constructing an early liver cancer rat model, comprising the following steps:

[0046] Rats with a body weight of 180-220 g were allowed to freely drink a diethylnitrosamine solution with a concentration of 60 mg / L (prepared by sterilized water), which was changed every day according to the drinking situation, and the rat model of liver cancer was obtained after drinking until the 10th week.

[0047] An evaluation method for rat models of early liver cancer, which is evaluated by the changes of endogenous metabolites in the body's end product urine before and after modeling, and the metabolic profile is characterized by multivariate statistical analysis, and principal component analysis (PCA) is used to analyze The data is pattern recognized, and the separation of the contours of each group of data is inspected, specifically:

[0048] In the 8th week, 10th week, and 12th week of rat model construction, the urine of blank and model group rats were collected ...

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Abstract

The invention discloses an early liver cancer rat model construction and evaluation method, and belongs to the technical field of model construction and evaluation methods. The scheme of the inventionmainly solves the problems of poor accuracy, low sensitivity, time waste and energy waste of a current early liver cancer rat model construction and evaluation method. The scheme of the invention adopts a metabonomics technology, and analyzes changes in endogenous metabolites in urine, the end product of the body before and after modeling, to obtain a dynamic trajectory map of the metabolic profile of the pathological process. In addition, all nuclear magnetic resonance spectrums are processed by using MestReNova software to obtain integral data, the content of 19 biomarkers is combined for statistical analysis, and results show that the change of the integral mean value of the 19 biomarkers in the urine before and after modeling reflects the change trend of the urine metabolic process ofrats with liver cancer. The method of the invention provides a detection method that is simple to obtain specimens, and is more economical, safe and effective, and provides a high-sensitivity, high-throughput, non-invasive, and highly-specific evaluation method for new drug research and development and pharmacological research.

Description

technical field [0001] The invention belongs to the technical field of model construction and evaluation methods, and in particular relates to a construction and evaluation method of an early liver cancer rat model. Background technique [0002] Liver cancer is one of the malignant tumors with the highest mortality rate in the world, and its mortality rate has been increasing year by year in the past 20 years. my country is one of the countries with a high incidence of liver cancer, and its incidence ranks second in the incidence of malignant tumors in my country. Liver cancer is a cancer caused by uncontrolled growth of liver cells caused by viruses, chemical carcinogens and many other factors. However, the exact etiology and pathogenesis of liver cancer are not yet fully understood. [0003] At present, the judgment of the success of the primary liver cancer model replication in rats is mainly based on the histopathological examination, the detection of serum alpha-fetop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N24/08
CPCG01N24/087G01N33/5091
Inventor 高丽王珂欣杜冠华秦雪梅
Owner SHANXI UNIV
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