Application of taiwanin H in preparation of herbicide
A herbicide and formulation technology, applied in the field of pesticide research and development, can solve the problems of phytotoxicity of subsequent crops, enhanced weed resistance, slow degradation of chemical herbicides, etc., and achieve significant effects.
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Embodiment 1
[0029] Embodiment 1 The separation of compound Taiwanin H
[0030] After drying and pulverizing the stem bark of the plant, it is extracted with methanol and concentrated to obtain the methanol extract. After the extract was dissolved in methanol and suspended in water, it was sequentially extracted with petroleum ether and ethyl acetate to obtain the ethyl acetate layer extract.
[0031] The ethyl acetate extract was subjected to silica gel column chromatography with chloroform / methanol system, and then combined by TLC. Combined with activity tracking, the active part was subjected to silica gel column chromatography with petroleum ether / acetone, petroleum ether / ethyl acetate, and chloroform / ethyl acetate in sequence, and then separated by Sephadex LH-20 Sephadex LH-20, after purification , available from Taiwanin H.
Embodiment 2
[0032] The herbicidal activity of embodiment 2 Taiwannin H
[0033] The herbicidal activity of Taiwanin H on different types of weeds was determined by the small cup method, and the results are shown in Table 1.
[0034] Table 1 Herbicidal activity of Taiwanin H (50mg / L, 7d)
[0035] weed species Root length inhibition rate (%) Fresh weight inhibition rate (%) Red thistle (Ageratum conyzoides L.) 100.00±0.00 88.98±3.22 Mikania micrantha Kunth 100.00±0.00 81.02±0.34 Nightshade (Solanum nigrum L.) 100.00±0.00 69.78±1.66 Purslane (Portulaca oleracea L.) 100.00±0.00 71.02±0.65 Amaranth (Amaranthus retroflexus L.) 100.00±0.00 70.52±3.65 Lychee Grass (Saluia plebeia R.Br) 100.00±0.00 63.92±1.19 Plantain (Plantago asiatica L.) 100.00±0.00 68.50±0.79 Water lotus grass (Drymaria diandra Blume) 100.00±0.00 60.11±1.10 Duckweed (Lemna minor L.) 93.86±1.46 75.23±0.65 Amaranth (Amaranthus lividus L.) 91.88±1.03...
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