Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, and method and purpose

A bio-organic fertilizer and tomato bacterial wilt technology, applied in the field of antagonistic actinomycetes and bio-organic fertilizers, can solve problems such as difficult survival, achieve the effects of large quantity and low price, reducing the occurrence of bacterial wilt and protecting the environment

Active Publication Date: 2019-12-20
BIOGAS SCI RES INST MIN OF AGRI
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Problems solved by technology

[0004] The invention provides an antagonistic actinomycete for preventing and controlling tomato bacterial wilt, a bio-organic fertilizer and its method and use. The bio-organic fertilizer solves the problem that the existing bio-control bacteria are difficult to survive after being inoculated into the roots of plants, and can colonize In the rhizosphere of plants, and produce antagonistic substances, inhibit the growth of pathogenic microorganisms in the rhizosphere of plants

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  • Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, and method and purpose
  • Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, and method and purpose

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Embodiment 1

[0023] 1. Isolation and identification of strains

[0024] Collect tomato planting fields in Chengdu Shuangliu Biogas Science Research Institute Base of the Ministry of Agriculture, select healthy tomato plant rhizosphere soil around the diseased tomato plant to isolate actinomycetes, and use PDA medium (potato dextrose agar medium) to screen the tomato by confrontation method. Antagonistic actinomycetes that have a strong inhibitory effect such as the pathogenic bacteria R. solanacearum of soil-borne root diseases. After re-screening in the pot test, the highly effective antagonistic actinomycete G33 strain was finally obtained, and the biological identification of the strain was carried out. The strain was stored in a 4°C refrigerator and preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation number is CGMCC4.7604, the preservation time is July 15, 2019.

[0025] Antagonistic actinomycete G33 belon...

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Abstract

The invention discloses an antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, and a method and a purpose. The antagonistic actinomycetesG33 are preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.4.7604, the preservation date is 15 July 2019, and the antagonistic actinomycetesbelong to streptomyces microflavus. The antagonistic actinomycetes, biogas residues, dairy manure and rice straw are fermented, and the biological organic fertilizer is prepared. The biological organic fertilizer can improve soil microflora, and the antagonistic actinomycetes G33 can be well colonized in tomato rhizospheres, can produce antagonistic substances, and can restrain growth of pathogenic microorganisms at the tomato rhizospheres, so that the generation of tomato bacterial wilt caused by continuous cropping can be notably reduced, and the preventing and controlling rate reaches 70%or above.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain, in particular to antagonistic actinomycetes for preventing and controlling tomato bacterial wilt, biological organic fertilizer, method and application. Background technique [0002] The mechanism of microbial control of crop diseases is mainly composed of competition, antagonism, quorum sensing inhibition, induction of resistance to plants, and growth promotion. Many studies have shown that the survival and successful colonization of biocontrol bacteria in the plant rhizosphere is the key The key to controlling crop diseases. The traditional biological control technology is to spray the bio-control bacteria into liquid or powder bacterial agents, which can effectively prevent and control the reproduction and invasion of pathogenic bacteria. Harsh conditions make it difficult to colonize and even survive, resulting in unstable control effects and poor sustainability. Therefore, there are certain...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20C05G3/00A01P3/00C12R1/465
CPCC05F3/00C05G3/00C12N1/20C12N1/205C12R2001/465C05F9/04C05F11/00C05F11/08Y02W30/40
Inventor沈婷雷云辉张思洋
OwnerBIOGAS SCI RES INST MIN OF AGRI