Supercharge Your Innovation With Domain-Expert AI Agents!

A new method and application of producing ferulic acid esterase by fermenting straw of Pyrocystis sp.

The technology of ferulic acid esterase and Trichoderma piriformis is applied in the field of anaerobic fermentation of Leymus chinensis to produce ferulic acid esterase, which can solve the problems of low conversion efficiency, long pretreatment time and high cost, and achieves improved economic benefits, Simple fermentation process and low equipment requirements

Inactive Publication Date: 2020-08-04
BAIYIN SINO BIOTECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anaerobic digestion, but these pretreatment methods are costly and not environmentally friendly
Ordinary microbial treatment also has many defects. The effect of single microbial treatment is not good, and the effect of composite bacterial flora of artificial components is not ideal. There is mutual antagonism between various bacterial species, resulting in long pretreatment time and low conversion efficiency. A complete protocol for anaerobic fermentation of Leymus chinensis to produce ferulic acid esterase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A new method and application of producing ferulic acid esterase by fermenting straw of Pyrocystis sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of piromyces Piromyces CY1 inoculum:

[0034]Draw 1mL of Piromyces CY1 culture and inoculate it into a 20mL Heinz anaerobic tube with 9mL of liquid basic medium based on 0.1g of air-dried and crushed wheat straw. The final concentrations of chloramphenicol in the liquid basic medium were 1600IU / mL and 2000IU / mL respectively, and the final concentration of chloramphenicol in the medium was 50 μg / mL. After anaerobic culture at 39°C for 72 hours, the growth peak is reached, and the fermentation broth is a high-activity bacterial agent at this time.

Embodiment 2

[0035] Example 2: Production of ferulic acid esterase by anaerobic fermentation of Leymus chinensis by Piromyces CY1

[0036] 1. The method for producing ferulic acid esterase by anaerobic fermentation Leymus chinensis

[0037] 90mL of liquid basic medium was placed in a 100mL anaerobic fermentation bottle, and 1.0g of crushed and air-dried Leymus chinensis, rice straw, wheat straw and corn straw were used as substrates. Deoxygenation. Sterilize. Inoculate 10 mL of Piromyces CY1 subcultured for 72 hours into the above-mentioned anaerobic medium with Leymus chinensis, rice straw, wheat straw and corn straw as substrates, and compound antibiotics were added at the same time to make it The final concentration of penicillin 1600IU / mL and streptomycin sulfate 2000IU / mL in the anaerobic medium solution, the final concentration of chloramphenicol in the medium was 50 μg / mL, and anaerobic culture was carried out at 39°C for 7 days. A total of 3 parallel experiments were set up to m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of renewable energy sources of biotechnology, in particular to Piromyces CY1, a method for producing feruloyl esterase by fermenting straw with the Piromyces CY1, and application of the Piromyces CY1. The invention discloses a method for producing feruloyl esterase by carrying out anaerobic fermentation on straw with the Piromyces CY1. The Piromyces CY1 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the Piromyces CY1 is CGMCC NO. 18141. The Piromyces CY1 disclosed by the invention can be preserved in vitro for passage and survival. Fermented straw can produce the feruloyl esterase with high activity. The fermentation process is simple, low in equipment requirement and convenient to popularize, and has important industrial application value and development prospect in the industrial field.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a method for anaerobically fermenting Leymus chinensis to produce ferulic acid esterase. Background technique [0002] Leymus chinensis is an eastern meadow in the steppe region of Eurasia grassland It is distributed in a wide range from 36°N to 62°N and 120°E to 132°E, accounting for more than half of it in China. Leymus chinensis grows well in cold and dry areas. It turns green early in spring and turns yellow late in autumn. It can provide more green fodder for a long time, and the accompanying quantity produced every year is also very huge. Leymus chinensis has not been rationally developed and utilized as an energy source. The existing anaerobic digestion technology has the problems of low technical efficiency and great difficulty in popularization. [0003] Lignocellulose is the main component of Leymus chinensis, and the hydrolysis of lignocellulose is the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/18C12N1/14C12R1/645
CPCC12N1/14C12N9/18C12Y301/01073
Inventor 魏亚琴王治业杜军国祁宏山张静荣
Owner BAIYIN SINO BIOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com