A small multi-angle scanning surface plasmon resonance biochemical analyzer

Abstract

The invention discloses a small multi-angle scanning surface plasma resonance biochemical analyzer, which comprises a horizontal transmission module, a symmetrical optical module, a prism and flow pool module, a vertical transmission module, an instrument environment control module and a control and communication module. A motor is used to rotate to drive a belt, the belt pulls two connecting rods, and the length and angle of the mechanical connecting rods of incident light and reflected light are adjusted at the same time, and the position of the prism remains unchanged. Thereby changing the incident angle and reflection angle to produce a symmetrical motion angle adjustment system. The fine-tuning structure for changing the position of parts on the incident connecting rod and reflecting connecting rod of the present invention meets the needs of incident and reflected light paths of prism light sources of different shapes. The present invention also designs a structure in which the pressing piece cooperates with the pressure regulating piece so that the force on the flow cell is even. It has the characteristics of simple structure, high precision, wide angle detection range, high angle resolution, and is not limited by the machining accuracy of mechanical parts.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis and detection, and realizes label-free quantitative biochemical analysis of detection objects by detecting surface plasmon resonance phenomena. Background technique [0002] Surface plasmon resonance technology, abbreviated as SPR in English, is a new technology developed in recent years. It uses the principle of resonance between evanescent waves and surface plasmon waves to detect the interaction between molecules and analytes on biosensor chips. In this case, the change process of the optical parameters of the sensing medium is detected. [0003] Compared with traditional biochemical analysis technology, SPR sensing technology, as a surface detection technology, has the advantages of high sensitivity, label-free, real-time rapid and non-destructive detection, etc., and has been widely used in life sciences, medical testing, drug screening, Food testing, environmental monitoring, d...

AI Technical Summary

Problems solved by technology

[0006] There are two ways to achieve angle modulation. One is that the incident light is parallel light, and the position of the incident light source and receiver is changed through a mechanical mechanism, so as to scan the curve of light intensity changing with the incident angle. This method detects the angle Resolution is limited by machining accuracy of mechanical parts

Another method is the converging beam method. The incident light is a beam of non-parallel converging light, which converges on the sensitive chip in a certain angle range, and becomes a beam of divergent light after reflection. The light intensity of the detection beam changes with the divergence angle. The angle modulation curve of SPR can be obtained. This method avoids the influence of the precision of the mechanical scanning mechanism and can obtain higher precision, but the detection range is small

[0007] However, it is worth noting that even GE’s BIACORE series, which occupies an absolute dominant position in the SPR market, their products are still mainly aimed at scientific research institutes and laboratories; BIACORE devices are bulky, expensive, and fixed-angle Detection, unable to realize simultaneous detection of gas phase and liquid phase, not suitable for outdoor monitoring

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