Bactericide composition
A bactericide composition and alkyl technology, which can be applied in biocides, disinfectants, animal repellents, etc., can solve the problems of inability to obtain bactericidal effect and low bactericidal effect, and achieve excellent bactericidal effect and excellent bactericidal effect
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[0086] The fungicide compositions shown in Tables 1 to 4 were prepared, and the following evaluations were performed. The pH value is 7 at 20°C. The results are shown in Tables 1-4.
[0087] (1) 10 mL of LB medium was placed in a tissue culture flask, and bacteria (Staphylococcus aureus or Escherichia coli ) cultured in a petri dish were taken out through a platinum loop and placed in LB medium.
[0088] (2) The above-mentioned flask was incubated at 200 rpm and 37° C. for 16 hours.
[0089] (3) Transfer the above-mentioned LB medium to a 50 mL centrifuge tube, and perform centrifugation at 4000 rpm, 15° C., for 10 minutes.
[0090] (4) The LB medium in the supernatant was discarded, 10 mL of physiological saline was added, and centrifugation was performed again under the same conditions as in (3).
[0091] (5) The physiological saline in the supernatant was discarded, and physiological saline was added to prepare a bacterial solution with an OD (optical density) of 1.
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