Ratio electrochemical detection method of ochratoxin A

An ochratoxin and detection method technology, applied in the field of biosensing, can solve the problems of electrochemical sensors being easily interfered by external factors, low reliability accuracy, insufficient accuracy, etc., and achieves high reliability, improved reliability and high reliability. Accuracy and high sensitivity
CN110618185AActive Publication Date: 2019-12-27JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2019-12-27

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Abstract

The present invention belongs to the field of biosensing technologies, and relates to a method for preparing a ratio electrochemical aptamer sensor for detecting ochratoxin A, and the method is used for detecting the ochratoxin A (OTA). The ratio sensing policy is constructed by sequentially modifying ferrocene marked complementary DNA (Fc-cDNA), ochratoxin A aptamer (Aptamer) and auxiliary complementary DNA (hDNA) on a gold electrode, double current signals IFc and IMB (which are respectively oxidized electric currents of Fc and MB) are output by using a target-induced comformational change,and OTA is detected by using a ratio signal (IFc/IMB) quantization sum of IFc and IMB. The obtained ratio electrochemical aptamer sensor can implement highly sensitive and highly reliable detection onthe OTA, a detection linearity range is 10 pg/mL to 10 ng/mL, and a detection limit is 3.3 pg/mL. The present invention aims to develop a ratio electrochemical aptamer sensor that is high in sensitivity, good in selectivity, and high in reliability, and provide a reliable sensing platform for measuring OTA in an actual sample.
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Claims

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