Seed preservation technology of immobilized microalgae by using organic synthetic polymers

Abstract

The present invention provides a seed preservation technology of immobilized microalgae by using organic synthetic polymers in the technical fields of seed preservation. Firstly, an organic syntheticpolymer fixative, sodium chloride and algae liquid are quantitatively weighed, then the organic synthetic polymer fixative, sodium chloride and algae liquid are separately added into distilled water to prepare a gel solution of a certain concentration, the organic synthetic polymer fixative gel solution is boiled and disinfected, then the organic synthetic polymer fixative gel solution is fully and evenly mixed with the algae liquid in a certain amount, the mixture is evenly dropped into a CaCl2 solution at a uniform rate by a syringe, dropping is conducted while shaking is conducted, after acertain period of time, solidified microalgae beads are formed, the formed calcium micro-alginate beads are washed several times with sterile distilled water to obtain fixed algal cells, the fixed algal cells are preserved and cultured, finally, timing sampling for a resuscitation test is conducted, culture at room temperature is conducted for 10 days, and first visual inspection and then microscopic inspection are conducted to determine whether the algal cells are alive. The preparation method is simple, liable to operate, not liable to contaminate, and longer in seed preservation time.

Description

[0001] technology neighborhood [0002] The invention relates to a seed preservation technology, in particular to a technology of immobilizing microalgae seed with organic synthetic macromolecule. Background technique [0003] Microalgae are rich in nutrients and have a wide range of applications in people's lives and production practices. In the cultivation and application of microalgae, species preservation technology is very important. The large-scale microalgae industry is not enough to cultivate microalgae alone. The algae species must be preserved for later use to meet the needs, but the individual microalgae are small and difficult to separate, and it is easy for the single-cell pure species of the algae species to mutate with environmental changes during the species preservation process, losing their own physiological activity and genetic characteristics, so Preservation of algae species is particularly important. At present, the method of preserving algae species ofte...

AI Technical Summary

Problems solved by technology

[0003] Microalgae are rich in nutrients and have a wide range of applications in people's lives and production practices. In the cultivation and application of microalgae, species preservation technology is very important. The large-scale microalgae industry is not enough to cultivate microalgae alone. The algae species must be preserved for later use to meet the needs, but the individual microalgae are small and difficult to separate, and it is easy for the single-cell pure species of the algae species to mutate with environmental changes during the species preservation process, losing their own physiological activity and genetic characteristics, so The preservation of algae species is particularly important. At present, the method of preserving algae species often adopts the method of subculture, which is repetitive and cumbersome.