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Application of cymbidium hookerianum extract

An extract, the technology of tiger head orchid, applied in the application field of tiger head orchid extract, can solve the problems of reduced adhesion, accelerated skin aging and other problems, to promote proliferation, promote cell repair or promote skin surface repair, The effect of simple preparation process

Pending Publication Date: 2020-01-07
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increase of age and the influence of adverse environmental factors such as external ultraviolet radiation, the skin protrusions at the junction of the dermis and the epidermis gradually flatten and cause overlap and breakage at the junction of the dermis and the epidermis, which not only leads to a decrease in the adhesion between the dermis and the epidermis , and the reduction in the delivery of nutrients between the dermis and the epidermis leads to the acceleration of skin aging

Method used

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  • Application of cymbidium hookerianum extract
  • Application of cymbidium hookerianum extract
  • Application of cymbidium hookerianum extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The production of embodiment 1 tiger head orchid extract

[0044] Take 50-200 mesh tiger head orchid (whole plant) dry powder 10g with a water content lower than 5%, and extract 2 times with 70% (volume concentration) ethanol 500W ultrasonic extraction at room temperature, each time with 100mL 70% extraction for 30min, the filtrate Combined, the filtrate was rotary evaporated to dryness to obtain about 1.0 g of Sansevieria extract. And evaluate the efficacy of tiger head orchid extract.

Embodiment 2

[0045] Embodiment 2 3D skin model test

[0046] (1) Preparation of skin model

[0047] Primary human fibroblasts (NHDF) and primary human keratinocytes (NHEK) were extracted from skin tissue. Resuscitate primary human fibroblasts, culture and digest them and mix them with collagen for 3 to 4 days in the dermis stage, then digest and inoculate the primary human keratinocytes cultured on the feeder layer on the surface of the skin model in the dermal stage, and pass through the epidermis in turn In the immersion culture and gas-liquid phase culture phases, culture to 18 days to obtain a 3D skin model that can be used for detection.

[0048] During the culture, from D3 to the final D18 after the fibroblasts were inoculated on the scaffolds, ordinary culture medium (control group, NT) or containing 10 μg / mL of Example 1 tiger head orchid extract (about 0.001wt%, in the form of The skin model was cultured in the culture medium of the dry weight of the tiger head orchid extract. ...

Embodiment 3

[0053] Example 3 Tiger head orchid extract improves skin cell repair / proliferation-related gene expression

[0054] (1) The preparation method of the skin model is the same as in Example 2.

[0055] (2) Extraction of RNA

[0056] Cut the tissue about 2*3mm, put it into a 2mL EP tube; add 1mL Trizol, add 2 steel beads, homogenize with a tissue disruptor, 50Hz, 5min; 9000rpm, 4°C for 1min, transfer the supernatant to a new tube Into a 1.5mL EP tube; add 200uL chloroform, gently invert several times to mix, and place at room temperature for 5 minutes; 9000rpm, 4°C for 15min; transfer the upper aqueous phase (about 400uL) to a new 1.5ml EP tube, add an equal volume of isopropyl Alcohol, mix well, let stand at room temperature for 10 minutes; 9000rpm 10min 4 ℃; discard the supernatant, wash the precipitate with pre-cooled 70% absolute ethanol once, 8000rpm 5min 4 ℃; discard the supernatant, air dry for 5-10 minutes, dissolve In 30ul DEPC water; spectrophotometer to measure RNA co...

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PUM

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Abstract

The invention relates to the fields of daily chemical and plant extract, in particular to application of cymbidium hookerianum extract. The cymbidium hookerianum extract has the ability to improve theexpression of cell repair / proliferation-related genes and basement membrane-related genes, and can improve skin cell repair capacity, increase epidermal thickness, stabilize dermal-epidermis junctionand stabilize skin basement membranes. Therefore, the cymbidium hookerianum extract can be used for preparing skin external preparations and used as an anti-aging active ingredient for maintaining skin stability.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to the application of tiger head orchid extract. Background technique [0002] The skin covers the entire human body and is in direct contact with the external environment. In addition to protecting the body from various external factors, the skin is also a mirror that reflects the health of the whole body. Skin is composed of epidermis, dermis, and subcutaneous tissue, and contains skin appendages, abundant nerves, blood vessels, lymphatic vessels, and muscles. [0003] The connection area between the dermis and the epidermis is also called the basement membrane zone. The basement membrane is the support pad of the epithelial cells. It has the function of supporting and connecting between the epithelial cell tissue and the connective tissue. material exchange. The basement membrane not only serves as a structural model to support epidermal cells, but also serves as a selective barrier fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61Q19/08A61Q19/00
CPCA61K8/9794A61Q19/08A61Q19/00A61K2800/78
Inventor 吴越宋肖洁姜丹姚期凤王越蒋耀权章漳
Owner JALA GROUP CORPORATION
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