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Enteric microorganism markers highly related to anorexia actions of small-weight chickens

A technology of intestinal microbes and markers, which is applied in the direction of microbes, microbe-based methods, microbiological measurement/testing, etc., and can solve problems affecting economic benefits, obesity, and affecting normal egg production of hens

Active Publication Date: 2020-01-07
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the poultry farming industry, overfat hens can affect the normal egg production of hens to a large extent and affect economic benefits

Method used

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  • Enteric microorganism markers highly related to anorexia actions of small-weight chickens
  • Enteric microorganism markers highly related to anorexia actions of small-weight chickens

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Experimental program
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Effect test

Embodiment 1

[0032] 1) Long-term artificial difference selection was carried out with the white Roc chicken as the common ancestor, aiming at the height of body weight at 56 days old, to ensure that the feeding conditions of body weight and the feeding environment were consistent, so as to obtain the difference of a single trait, and selected 56 After one generation, the obtained chickens of the small body weight family have obvious symptoms of anorexia and emaciation, and the average body weight is only about one-tenth of the weight of the large body weight family. 5 hens each (1976±193g for large body weight roosters; 1598±166g for large body weight hens; 174±46g for small body weight roosters; 128±37g for small body weight hens). The DNA of the intestinal flora was extracted after the sample, and the specific steps were as follows: Microbial DNA extraction was carried out in strict accordance with the instructions of the product kit (TIANGEN DNA stool mini kit). After the microbial DNA ...

Embodiment 2

[0039] 1) Data set creation

[0040] Select the gut microbes with differences in the abundance obtained from the analysis of the small-weight chicken family and the large-weight chicken family, and remove redundant or irrelevant gut microbes; use 2 / 3 of the total number of samples as the training set, and 1 of the total number of samples / 3 as a verification set, import the data into R language software (v3.6.1), and use the randomforest package (v4.6) to construct a random forest model.

[0041] 2) Train the model and verify

[0042] Import the verification set into the constructed random forest model, and find that the prediction error rate is zero, indicating that the model is reliable. Export the important variables involved in model construction. It was found that the MeanDecreaseGini values ​​of Rhodovulum bhavnagarense strains and Terrisporobacterglycolicus strains (influence values ​​on the accuracy of model classification effects) were relatively large, respectively...

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Abstract

The invention relates to enteric microorganism markers highly related to anorexia actions of small-weight chickens. The markers respectively include a Rhodovulum bhavnagarense strain belonging to Rhodovulum and a Terrisporobacter glycolicus strain belonging to Terrisporobacter. The markers are enriched in intestinal tracts of the small-weight chickens, and the chickens show anorexia, and are thinand small. The discovering of the markers provides reference for research and development of microorganism weight-reducing products. In addition, the enteric microorganism markers can be used for controlling the weight of egg-laying chickens in production of poultry.

Description

technical field [0001] The invention relates to the field of microbial markers, in particular to an intestinal microbial marker highly correlated with anorexia behavior of small body weight chickens. Background technique [0002] In the poultry farming industry, overfat and overweight hens can affect the normal egg production of hens to a large extent and affect economic benefits. At present, obesity is also an important cause of human cardiovascular and cerebrovascular diseases, cancer, diabetes and other chronic diseases, and is listed by the World Health Organization as one of the top ten diseases that threaten human health. Over the past 20 years, with the improvement of living standards, Chinese people have become "fat" at an astonishing rate, and obesity has become a serious social problem. Therefore, it is increasingly difficult to find healthy and feasible methods to control obesity. [0003] It is well known that the diversity, composition, and functional aspects ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/689C12R1/01
CPCC12Q1/04C12Q1/689G01N2333/195G01N2800/7066
Inventor 周浩孟和杨凌宇何川丁金梅徐珂郑煜明韩铖潇罗怀希
Owner SHANGHAI JIAO TONG UNIV
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