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A method for the purification and separation of artemisinin enhanced by chitosan functional membrane

A technology of purification and separation, chitosan, applied in chemical instruments and methods, ion exchange, organic chemistry, etc., can solve the problems of complicated separation steps, achieve efficient purification and separation methods, improve purity, and strengthen the effect of purification and separation process

Active Publication Date: 2020-11-20
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still requires more than two recrystallizations during separation, and the separation steps are relatively complicated.

Method used

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  • A method for the purification and separation of artemisinin enhanced by chitosan functional membrane
  • A method for the purification and separation of artemisinin enhanced by chitosan functional membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Dissolve 1g of extract in 7mL of 70% aqueous methanol, stir at 55°C for 0.5h, filter to obtain an extract containing artemisinin and wax oil, then add 0.2g of C 8 Modified chitosan functional membrane, stirred at 30°C for 2h, placed in a low-temperature constant temperature tank, crystallized at 25°C for 1h, filtered, and dried at 50°C, the HPLC spectrum of the obtained product is as follows: figure 1 As shown, the peak eluting time is 14.048min, and the purity of artemisinin is 98.4%.

[0055] Wherein, the preparation method of modified chitosan functional film is: add 5mL tetraethyl orthosilicate and 5mL n-octyltrimethoxysilane in 85mL ethanol, the volume ratio of tetraethyl orthosilicate and ethanol is 1:17, stir 0.5h; after adding 0.1g of chitosan functional film, quickly add a mixed solution containing 5mL of ammonia water and 35mL of water. The mass concentration of ammonia water used in the experiment was 28%, stirred at room temperature for 4h, washed with ethan...

Embodiment 2

[0057] Dissolve 1g of extract in 7mL of 70% aqueous methanol, stir at 55°C for 0.5h, filter to obtain an extract containing artemisinin and wax oil, then add 0.03g of chitosan functional membrane, stir at 50°C for 2h, and place Crystallize in a low-temperature constant temperature bath at 10°C for 5 hours, filter, and dry at 50°C. The peak position similar to that of Example 1 was obtained by HPLC detection, the peak time was 14.041min, and the purity was 86.8%.

Embodiment 3

[0059] Dissolve 1g of extract in 7mL of 70% aqueous methanol, stir at 55°C for 0.5h, filter to obtain an extract containing artemisinin and wax oil, then add 0.2g of C 8 Modified chitosan functional film, stirred at 30°C for 2h, then placed in a low-temperature constant temperature tank for crystallization at 10°C for 1h, filtered, and dried at 50°C, the HPLC spectrum of the product obtained obtained a peak position similar to that of Example 1, and the peak time It was 14.079min, and the purity of artemisinin was 100%.

[0060] Wherein, the preparation method of the modified chitosan functional film is the same as that of Example 1.

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Abstract

The invention relates to a method for strengthening purification and separation of artemisinin by a chitosan functional membrane. An extractant is added to an extract containing artemisinin and wax oil to obtain an extraction solution, the chitosan functional membrane or a modified chitosan functional membrane is added to the extraction solution, and separation and purification are performed to obtain artemisinin. The chitosan functional film or the modified chitosan functional film can adsorb the wax oil in the extraction solution, and the wax oil is easily separated from artemisinin after being adsorbed, so that high-purity artemisinin is obtained. At the same time, the chitosan functional film or the modified chitosan functional film has simple preparation method, and abundant and non-toxic and harmless raw materials. The method can also reduce a number of crystallization times and a crystallization time required in a process of obtaining an artemisinin product, and is an efficientpurification and separation method.

Description

technical field [0001] The invention relates to the field of natural product extraction, in particular to a method for extracting artemisinin, and in particular to a method for strengthening the purification and separation of artemisinin with a chitosan functional membrane. Background technique [0002] Artemisinin or Qinghaosu (QHS) is a new type of sesquiterpene lactone drug derived from the plant Artemisia annua L., which is called "the only effective malaria treatment drug in the world" by the World Health Organization. It can directly kill malaria parasites, and has the characteristics of quick action and low toxicity for the treatment of chloroquine falciparum malaria. In addition, recent studies have found that artemisinin also has pharmacological effects such as anti-schistosomiasis, anti-tumor, treatment of diabetes, kala-azar, and lupus erythematosus. Therefore, studies on the physical and chemical properties, extraction, separation and detection methods, pharmaco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/20B01D15/08B01J20/24B01J20/30
CPCB01D15/08B01J20/24C07D493/20
Inventor 王慧曹莹莹张迎张永强张锁江
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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