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Antibacterial nanoprobe BSA@AuNc-AMP-Ce6 and preparation method and application thereof

A nanoprobe and antimicrobial peptide technology, which is applied in the field of new nanoprobe BSA@AuNc-AMP-Ce6 and its preparation, can solve the problem of ineffective bactericidal effect, and achieve suitable for popularization, wide application and high antibacterial activity Effect

Inactive Publication Date: 2020-01-17
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing photosensitizers are loaded on nanomaterials, and only relying on photosensitizers for sterilization, the bactericidal effect is not obvious

Method used

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  • Antibacterial nanoprobe BSA@AuNc-AMP-Ce6 and preparation method and application thereof
  • Antibacterial nanoprobe BSA@AuNc-AMP-Ce6 and preparation method and application thereof
  • Antibacterial nanoprobe BSA@AuNc-AMP-Ce6 and preparation method and application thereof

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Embodiment 1

[0052] 1. Synthesis and purification of nanoprobe BSA@AuNc-AMP-Ce6 (Ce6-GKRWWKWWRRPLGVRGC-BSA@AuNc)

[0053] 1) Synthesis and characterization of BSA@AuNc

[0054] Mix 5ml BSA (50mg / ml) with 5ml HAuCl 4 (10mM) respectively in 37°C water bath for 10min, then HAuCl 4 Added dropwise into BSA, and after 2 min, 500 μL of NaOH solution (1M) was added dropwise into the above solution, and then stirred at 37° C. for 12 h. Put the sample into a dialysis bag (12-14KD), dialyze in water for 20 hours, and finally disperse the solution in water. Note: All glassware should be washed and the water should be ultrapure water.

[0055] After the synthesized BSA@AuNc was filtered through a water system (0.22μ) filter membrane, it was diluted twice, each sample was measured in parallel three times, and the average value was taken, and the ultraviolet absorption of the synthesized BSA@AuNc was scanned with a UV spectrophotometer. The scanning range was 200nm~800nm, use a fluorescence spectrome...

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Abstract

The invention belongs to the technical field of metal nanomaterials, and particularly discloses an antibacterial nanoprobe BSA@AuNc-AMP-Ce6 and a preparation method and application thereof. An antibacterial peptide marked by Ce6 is connected to BSA@AuNc through an Au-S bond, and PLGVRG in a polypeptide sequence is a gelatinase enzyme digestion sequence and can be recognized and cut off by gelatinase secreted by S. aureus; the photosensitizer Ce6 is introduced for PDT antibiosis, and the photosensitizer Ce6 generates reactive oxygen species (ROS) under the irradiation of laser, so microbial molecules are oxidized and cells are damaged and die; and the photosensitizer Ce6 and antibacterial peptide (AMP) generate a synergistic effect. The antibacterial nanoprobe BSA@AuNc-AMP-Ce6 prepared by using the preparation method disclosed by the invention has more excellent antibacterial performance than traditional antibacterial peptides, and is good in biocompatibility, small in interference to normal physiological activities of body cells, low in toxicity and high in safety.

Description

technical field [0001] The invention belongs to the technical field of metal nanomaterials, and in particular relates to a novel nanoprobe BSA@AuNc-AMP-Ce6 with antibacterial properties and photodynamic therapy and its preparation method and application. Background technique [0002] With the development of science and technology in recent years, due to the characteristics of quantum size effect, plasmon resonance effect and quantum tunneling effect, fluorescent nanomaterials show distinctive optical, chemical, electrical and catalytic properties, making them widely used in the scientific community. attention. The fluorescent nanomaterials that have been researched and discovered mainly include: organic dyes, carbon quantum dots, polymer dots, semiconductor quantum dots, fluorescent metal nanoclusters (FM NCs) and so on. At present, a variety of fluorescent metal nanoclusters have been discovered, such as platinum nanoclusters, gold nanoclusters, silver nanoclusters, copper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/16A01N37/46A01P1/00C09K11/06C09K11/02C09K11/58B82Y30/00B82Y40/00G01N21/64C12Q1/14A61K41/00A61P31/04C12R1/445
CPCA01N37/46A01N59/16A61K41/0071A61P31/04B82Y30/00B82Y40/00C09K11/025C09K11/06C09K11/58C12Q1/14G01N21/6428G01N2021/6439G01N2333/31
Inventor 王建浩郭倩倩贾文静淩惠王博智刘丽崔朋飞邱琳周舒文王璇巢胡翡马剑鹤
Owner CHANGZHOU UNIV
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