Preparation method and application of monoamine oxidase A inhibitor

Abstract

The invention discloses a preparation method and application of a monoamine oxidase A inhibitor. The preparation method comprises the steps: S1, Chinese prickly ash and an ethanol solution are placedin to a container, and an extract solution is obtained through heating reflux; S2, ethanol is removed after reduced-pressure concentration, and an aqueous solution is obtained; S3, the PH value is adjusted to be 2-3, and acid filtrate and precipitates are obtained through filtering and precipitation; S4, after the precipitates are dried, an extract of the precipitates are obtained; S5, the PH value of the acid filtrate is adjusted to be 9-10, and alkaline filtrate is obtained; S6, the alkaline filtrate is extracted through dichloromethane, dichloromethane extraction liquid is obtained, and after concentration and drying, a dichloromethane extract-phase extract is obtained; S7, the alkaline filtrate is extracted through n-butanol, n-butanol extraction liquid is obtained, and after concentration and drying, an n-butanol extract-phase extract is obtained; and S8, sulfuric acid is added into the alkaline filtrate to adjust the PH value to be neutral, and an extract of a mother solution isobtained. The monoamine oxidase A inhibitor can be applied to prevention and treatment of related diseases with the excessively-high monoamine oxidase A activity.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method and application of a monoamine oxidase A inhibitor. Background technique [0002] Zanthoxylum bungeanum is the dried and mature peel of Zanthoxylumbungeanum Maxim. Zanthoxylum bungeanum first appeared in the "Book of Songs", and its medicinal use first appeared in "Shen Nong's Herbal Classic". It is a traditional Chinese medicine, spice and seasoning agent, and is widely cultivated in China, Japan and Korea. Modern research shows that Zanthoxylum bungeanum contains a variety of chemical components: including alkaloids, lignans, fatty acid amides, flavonoids and terpenoids. [0003] People have found 25 kinds of amides from Zanthoxylum bungeanum, namely: α-sanshool, β-sanshool, γ-sanshool, hydroxy-α-sanshool, hydroxy-β-sanshool, hydroxy-γ- Sanshool. Other chemical components in Zanthoxylum bungeanum include triterpenoids, sterols, hydrocarbons, flavonoid glyco...

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Problems solved by technology

However, the nature of its toxicity has not been elaborated; in addition, whether Zanthoxylum bungeanum has an effect

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