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Breeding method of trichoderma harzianum high-yielding strain for preventing and controlling potato root rot

A high-yield strain and root rot technology, applied in the field of microorganisms, can solve problems such as limited inhibitory effect, achieve the effects of reducing workload, improving efficiency, and improving breeding efficiency

Pending Publication Date: 2020-01-17
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the inhibitory effect of Trichoderma harzianum strains through natural selection on the pathogenic fungus Fusarium spp. High-yielding strains of mold metabolites can improve the control effect on potato root rot and reduce production costs

Method used

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  • Breeding method of trichoderma harzianum high-yielding strain for preventing and controlling potato root rot
  • Breeding method of trichoderma harzianum high-yielding strain for preventing and controlling potato root rot
  • Breeding method of trichoderma harzianum high-yielding strain for preventing and controlling potato root rot

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Embodiment 1

[0039] The present embodiment provides a kind of breeding method for the high-yielding bacterial strain of Trichoderma harzianum that is used for preventing and controlling potato root rot, comprising the following steps:

[0040] Step 1, preparation of spore suspension: Inoculate the M-17 strain of Trichoderma harzianum on the slant of PDA medium, in dark conditions, after culturing at 25°C for 7 days, wash the slant of the strain with physiological saline, and break up with glass beads , remove the hyphae to obtain a suspension containing spores;

[0041] Step 2, ultraviolet mutagenesis: place the spore-containing suspension obtained in step 1 in a flat-bottomed petri dish, place it at a vertical distance of 30 cm from a 15W ultraviolet lamp, and simultaneously irradiate with magnetic stirring for 30 seconds to carry out ultraviolet mutagenesis;

[0042] Step 3, plate separation and screening: then dilute the mutagenized suspension in step 2 and spread it on a PDA medium pla...

Embodiment 2

[0049] The present embodiment provides a kind of breeding method for the high-yielding bacterial strain of Trichoderma harzianum that is used for preventing and controlling potato root rot, comprising the following steps:

[0050] Step 1, preparation of spore suspension: inoculate the M-17 strain of Trichoderma harzianum on the slant of PDA medium, and cultivate it at 25°C for 6 days in the dark, then wash the slant of the strain with physiological saline, and break it up with glass beads , remove the hyphae to obtain a suspension containing spores;

[0051] Step 2, ultraviolet mutagenesis: place the spore-containing suspension obtained in step 1 in a flat-bottomed petri dish, place it at a vertical distance of 30 cm from a 15W ultraviolet lamp, and simultaneously irradiate with magnetic stirring for 60 seconds to carry out ultraviolet mutagenesis;

[0052] Step 3, plate separation and screening: then dilute the mutagenized suspension in step 2 and spread it on a PDA medium pl...

Embodiment 3

[0059] The present embodiment provides a kind of breeding method for the high-yielding bacterial strain of Trichoderma harzianum that is used for preventing and controlling potato root rot, comprising the following steps:

[0060] Step 1, preparation of the spore suspension: inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and cultivate it at 26°C for 6 days in the dark, then wash the slant of the strain with physiological saline, and break it up with glass beads , remove the hyphae to obtain a suspension containing spores;

[0061] Step 2, ultraviolet mutagenesis: place the spore-containing suspension obtained in step 1 in a flat-bottomed petri dish, place it at a vertical distance of 30 cm from a 15W ultraviolet lamp, and simultaneously irradiate with magnetic stirring for 90 seconds to carry out ultraviolet mutagenesis;

[0062] Step 3, plate separation and screening: then dilute the mutagenized suspension in step 2 and spread it on a PDA m...

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Abstract

The invention discloses a breeding method of a trichoderma harzianum high-yielding strain for preventing and controlling potato root rot. The trichoderma harzianum high-yielding strain is obtained through breeding via the steps of ultraviolet mutagenesis on an original strain, inoculation into a fermentation bottle, filtering of fermentation broth, preparation of a culture plate, and resistance screening; according to the quantity determined through strain breeding, a strain with a big bacterium inhibition zone on pathogenic bacteria of fusaria of the potato root rot is preferably selected forseparation purification and passage; and the fast screening of the trichoderma harzianum high-yielding strain is completed. The breeding method of the trichoderma harzianum high-yielding strain provided by the invention has the advantages that the strain breeding efficiency can be effectively improved, so that the strain breeding period can be shortened; the strain breeding needs 6 to 8 generations of breeding before, and the strain breeding can be completed only through 3 to 4 generations now; and the time is shortened by a half. The strain screening efficiency and the excellent strain breeding accuracy are improved, so that an excellent strain can be provided for trichoderma harzianum fermentation; and reliable guarantee is provided in an aspect of preventing and controlling the potatotrichoderma harzianum root rot.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a method for breeding high-yielding Trichoderma harzianum strains for preventing and controlling potato root rot. Background technique [0002] Trichoderma is a kind of microorganism that widely exists in nature. Trichoderma belongs to the phylum Deuteromycota, Hyphospora, and the genus Trichoderma. Mold, Trichoderma harzianum, Trichoderma longibrachiata, etc. Among them, the mycelium of Trichoderma harzianum is slender and colorless, with separation and many branches. The conidiophores grow from the side branches of the mycelia, opposite or alternate, generally with 2 to 3 branches, and small conidia. Stem bottle-shaped or conical, conidia mostly spherical, spore wall with small warts, blue-green. [0003] As a biocontrol fungus, Trichoderma harzianum can be used to prevent plant diseases caused by pathogens such as Pythium, Rhizoctonia solani, Fusarium, Rhizopus niger, Cyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N15/01C12N1/14C12Q1/18A01P3/00C12R1/885
CPCC12N1/14C12N13/00C12N15/01C12Q1/18
Inventor 王喜刚郭成瑾沈瑞清焦杨张丽荣杨波
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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