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A method for detecting nitrite based on silver-coated gold nanoparticles

A technology of gold nanoparticles and nitrite, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation, low sensitivity, high detection limit, etc., and achieve the effect of high sensitivity

Active Publication Date: 2022-06-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the methods for detecting nitrite include diazotization coupling spectrophotometry, liquid chromatography, spectrophotometry, electrochemical method, titration method, etc. These methods have complex operation, high detection limit and low sensitivity. and other limitations

Method used

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  • A method for detecting nitrite based on silver-coated gold nanoparticles
  • A method for detecting nitrite based on silver-coated gold nanoparticles
  • A method for detecting nitrite based on silver-coated gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0065] Example 1 Preparation of gold nanoparticles

[0066] Step 1) Synthesis of spherical gold nanoparticles as seeds by sodium citrate reduction method:

[0067] To the 250mL three-necked flask containing 99mL ultrapure water, add 1mL of chloroauric acid solution with a mass concentration of 1%, place the three-necked flask in the middle of the oil bath, stir to boil while heating, and then add 1mL mass Sodium citrate with a concentration of 1%, continue to heat for fifteen minutes after the solution turns wine red until the solution does not fade, the wavelength is about 520nm, and the size is 13nm.

[0068] Step 2) After cooling the synthesized spherical gold nanoparticles, add them into a 100 mL glass bottle with a lid and store them in a refrigerator at 4°C.

[0069] Step 3) Preparation of large gold nanoparticles:

[0070] Add 200uL of chloroauric acid solution with a mass concentration of 1%, 2mL of seed solution, 200uL of sodium citrate solution with a mass concentr...

Embodiment 2

[0071] Example 2 Preparation of silver-coated gold nanoparticles

[0072] To 2mL of the gold nanoparticles synthesized in Example 1, 4uL of HCl (0.1M), 200uL of AA (0.1M), and 4uLAgNO were added in sequence. 3 (0.01M), silver-coated gold nanoparticles can be obtained, the particle size is 50±5nm, and the maximum absorption peak of the ultraviolet absorption spectrum is at 526nm.

Embodiment 3

[0073] Example 3 Detection of nitrite

[0074] A method for detecting nitrite based on silver-coated gold nanoparticles, comprising the steps of:

[0075] Step S1: Add appropriate amount of HCl, AA and AgNO to gold nanoparticles 3 , to prepare silver-coated gold nanoparticles, as in Example 2;

[0076] Step S2: the glass slide is soaked in MPTMS solution, silver-coated gold nanoparticle solution, and naphthalene ethylenediamine hydrochloride saturated solution in sequence, so as to realize MPTMS modification, silver-coated gold nanoparticle modification and naphthylethylenediamine hydrochloride on the slide glass. Amine modification;

[0077] Specifically, including:

[0078] In step S21, anhydrous ethanol and (3-mercaptopropyl) trimethoxysilane (MPTMS for short) are used to modify the glass slide, and the MPTMS solution reacts with the surface of the glass slide to form a surface with mercapto-depressed groups; after the modification is completed, use Rinse the slides wit...

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Abstract

The invention discloses a method for detecting nitrite based on silver-coated gold nanoparticles, comprising the following steps: adding appropriate amount of HCl, AA and AgNO to the gold nanoparticles 3 , to prepare silver-coated gold nanoparticles; the glass slides were sequentially soaked in MPTMS solution, silver-coated gold nanoparticles solution, and saturated solution of naphthaleneethylenediamine hydrochloride; The solutions are mixed to form a mixed solution A; the modified glass slide is immersed in the mixed solution A, after the reaction is completed, the glass slide is washed with ultrapure water and dried, and then the light intensity of the silver-coated gold nanoparticles is observed through a dark field microscope Changes detected in nitrite levels. The method for detecting nitrite provided by the silver-coated gold nanoparticles provided by the invention has the advantages of convenience, quickness, low detection limit, high sensitivity, good selectivity, and spatial resolution ability for detection.

Description

technical field [0001] The invention relates to the technical field of nitrite detection, in particular to a method for detecting nitrite by silver-coated gold nanoparticles based on plasma resonance energy transfer. Background technique [0002] Nitrite widely exists in the human environment. Nitrate in the human body can be reduced to nitrite under the action of microorganisms; in the food industry, it can be used as a color-retaining agent for meat products, and is often added to bacon and ham to increase its color. Makes it beautiful; also acts as a preservative. The color and taste of nitrite is similar to that of table salt, making it difficult to tell the difference. High doses of nitrite are highly toxic, affecting the functioning of red blood cells, leading to death due to hypoxia. Under cooking conditions, nitrite reacts with amino acids to form nitrosamines, which are highly carcinogenic; and nitrites are easily metabolized with human proteins to form amine comp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 许东安清潇吴伟董界周荣菊林亲录
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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