A kind of preparation method of clam oligopeptide

A technology of clams and oligopeptides, which is applied in the field of preparation of oligopeptides of clams, can solve the problems of increased viscosity, unfavorable operation, etc., and achieves the effects of improving the extraction rate and improving the biological activity.

Active Publication Date: 2021-10-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the good solvent properties of ionic liquids, ionic liquids are also used for peptide extraction research, but the viscosity of ionic liquids increases at low temperatures, which is very unfavorable for operation.
However, there is no research on the application of this binary system to peptide extraction.

Method used

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  • A kind of preparation method of clam oligopeptide

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Embodiment 1

[0029] A preparation method of clam oligopeptides, comprising the following steps:

[0030] (1) Wash the fresh clams, remove the shells, take the viscera, put them in water, stir and soak to remove impurities, then adjust the viscera of meretrix meretrix to neutrality, and then homogenate to prepare clam homogenate;

[0031] (2) The clam homogenate and the compound solvent are uniformly mixed according to the material-to-liquid ratio of 1:2 to obtain the soaking liquid, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 and γ-butyrolactone at a volume ratio of 1:1;

[0032] (3) Lower the temperature of the soaking solution to -50°C, and pass pulse electric field treatment into the soaking solution. The solution was kept at -50°C for 7 hours, and then rose to room temperature 23±2°C for 10 minutes;

[0033] (4) 0°C centrifugation step (3) treated soaking liquid 3 times, rotating speed 10000r / min, recovering the supernatant separated each time, then washin...

Embodiment 2

[0038] A preparation method of clam oligopeptides, comprising the following steps:

[0039] (1) Wash the fresh clams, remove the shells, take the viscera, put them in water, stir and soak to remove impurities, then adjust the viscera of meretrix meretrix to neutrality, and then homogenate to prepare clam homogenate;

[0040] (2) The clam homogenate and the compound solvent are uniformly mixed at a material-to-liquid ratio of 1:4 to obtain the soaking liquid, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0041] (3) Reduce the temperature of the soaking solution to -55°C, and pass pulse electric field treatment into the soaking solution. The pulse electric field treatment method is: the number of pulses is 200 times, the electric field strength is 18kV / cm, the pulse width is 60μs, and the pulse frequency is 1000Hz. The solution was kept at -55°C for 8 hours, and then rose to room temperature for 20 minut...

Embodiment 3

[0047] A preparation method of clam oligopeptides, comprising the following steps:

[0048] (1) Wash the fresh clams, remove the shells, take the viscera, put them in water, stir and soak to remove impurities, then adjust the viscera of meretrix meretrix to neutrality, and then homogenate to prepare clam homogenate;

[0049] (2) The homogenate of meretrix meretrix and the compound solvent are uniformly mixed according to the material-to-liquid ratio of 1:3 to obtain the soaking liquid, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0050] (3) Reduce the temperature of the soaking solution to -60°C, and pass pulse electric field treatment into the soaking solution. The pulse electric field treatment method is: the number of pulses is 260 times, the electric field strength is 20kV / cm, the pulse width is 70μs, and the pulse frequency is 1100Hz. The solution was kept at -60°C for 7 hours, and then rose to r...

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Abstract

The present invention relates to the technical field of extracting polypeptides from clams. In order to further improve the extraction rate of clam oligopeptides, the method of the present invention provides a method for extracting clam oligopeptides. The ionic liquid EMIMBF4 and γ-butyrolactone are in a volume ratio of 1: 1 The binary system obtained by compounding is applied to the preparation of clam oligopeptides, and a pulsed electric field is applied at low temperature to decompose clam visceral tissues, and then enzymatically hydrolyzed, separated, and purified under optimized enzymatic hydrolysis conditions to obtain clam oligopeptides. In the clam oligopeptide preparation method of the present invention, ionic liquid EMIMBF4 and γ-butyrolactone are mixed at a ratio of 1:1 to make a compound solvent, treated at low temperature in conjunction with a pulsed electric field, and then enzymatically hydrolyzed by alkaline protease to extract the obtained clam oligopeptide The efficiency is improved, and the biological activity is also improved to a certain extent, and the degreasing process of 0.1mol / L sodium hydroxide solution is omitted.

Description

technical field [0001] The invention relates to the technical field of extracting polypeptides from clams, in particular to a preparation method of clams oligopeptides. Background technique [0002] Meretrix meretrix is ​​mainly produced in the coastal area. It has high economic value, rich nutrition, delicious meat, and high therapeutic and medicinal value. Li Shizhen's "Compendium of Materia Medica" said that clams can cure "sores, boils, swollen toxins, eliminate accumulations, and relieve alcohol poisoning". It has obvious inhibitory effect, and also has obvious curative effect on diseases such as asthma, chronic bronchitis, goiter, and lymphatic tuberculosis. In the previous Chinese patent application CN201410624999.7, the inventor team of the present application provided a method for preparing clam oligopeptides. After the viscera of clams were homogenized, sodium hydroxide solution was degreased, alkaline protease was enzymatically hydrolyzed, and then chromatographi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/16C07K1/24C07K1/36
CPCC07K7/06C12P21/06
Inventor 杨最素唐云平余方苗黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
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