Soybean fermentation composition for skin wound healing and preparation method thereof

A technology of wound healing and composition, which is applied in the field of soybean fermented composition, and can solve problems such as difficult wound healing

Inactive Publication Date: 2020-02-07
萧敬皇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the present invention uses a new preparation method to provide a pharmaceutical composition for skin wound heali...

Method used

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  • Soybean fermentation composition for skin wound healing and preparation method thereof
  • Soybean fermentation composition for skin wound healing and preparation method thereof
  • Soybean fermentation composition for skin wound healing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1. Preparation method of soybean fermented liquid contained in the composition of the present invention

[0020] The present invention uses a refiner to refine non-defatted non-genetically modified soybeans, adds brown sugar and mineral salt to the soybean liquid with a ratio of soybeans to water of 1:16, heats it, and obtains a soybean liquid culture medium without filtering, which is used for microorganisms Use of probiotic culture. The soybean liquid medium was fermented with microorganisms of the genus Bifidobacterium, Bacillus, Lactobacillus, Lactococcus and Streptococcus respectively according to the lactic acid bacteria fermentation method ( Carry out liquid culture separately like fermenting or heterotrophic fermenting, then add the soybean liquid medium to carry out amplified culture, finally mix the various strains together, add the soybean liquid medium to carry out the final culture; The bag is used as a flat bag-type fermenting container, the fe...

Embodiment 2

[0025] Embodiment 2. Composition of the present invention is used for the animal experiment module of skin wound healing

[0026] In order to evaluate whether the soybean ferment liquid contained in the composition of the present invention has the effect of promoting skin wound healing, an experimental evaluation was carried out.

[0027] In this example, about 6 weeks old male ICR strain mice (purchased from Lesco Biotechnology Co., Ltd.) were used, 8 in each group, weighing about 30 to 35 grams, and given a 7-day environmental adaptation period, fed with mouse feed (Chow 5001) and water were provided to the mice to eat freely. The test grouping of this embodiment is as follows:

[0028] (1) FSG group: that is, the experimental group of the present invention, fermented soybean fermented gel prepared by adding excipients, Fermented Soybean Filtrate Gel (FSG).

[0029] (2) Vehicle group: the gel prepared by substituting water for fermented soybean liquid and adding the same a...

Embodiment 3

[0036] Example 3. Effects of the composition of the present invention on the histopathology of skin wounds

[0037] In the above experiment of Example 2, on Day 9, 2 animals in each group were sacrificed, and tissues within the wound area were collected for histopathological sectioning and analysis. (Such as image 3 )

[0038] Re-epithelialization (H&E staining) assessment (eg image 3 Row 1) is an important indicator of the skin epidermis in wound healing. The results showed that the results of the FSG group and the Positive group showed a better trend than that of the Vehicle group.

[0039] In terms of granulation tissue evaluation, a large number of proliferating microvessels, fibrous connective tissue, and inflammatory cells can be seen in the proliferative phase of wound healing. Therefore, the size of the granulation tissue area should have significance for wound healing. This test uses Masson Trichrome staining (such as image 3 The 2nd row) was used to distinguis...

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Abstract

The invention provides a medical composition for skin wound healing. Specifically, the composition is a soybean fermentation composition which is soybean fermentation liquid prepared through co-culture of soybean liquid and one or more microbial probiotics flora, wherein a microbial probiotics flora formation is a group constituted by microorganisms of bifidobacterium, lactobacillus, bacillus, lactococcus and streptococcus. Healing of skin wounds can be effectively promoted, and the problems of chronic wound healing and difficult wound healing are solved.

Description

technical field [0001] The invention provides a fermented soybean composition for skin wound healing and a preparation method thereof. Background technique [0002] The main functions of the skin include protection, excretion, secretion, absorption, heat regulation, accumulation, regulation of sensory cognition and immune processes, so when there are wounds on the skin, such as those caused by general trauma or bedsores, the skin will be affected. Adverse effects, and unable to perform its normal functions. [0003] In addition, it is well known that diabetic wounds are not easy to heal. Patients are prone to autonomic neuropathy, which leads to dry skin and easy cracking; patients are also prone to vascular disease, which leads to easy blockage of terminal blood vessels, poor circulation, and skin atrophy. . [0004] The product of soybean fermented by microorganisms can also be used as anti-active oxygen composition, reagent, food, cosmetics and medicine (such as Japanes...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P17/02A61P29/00
CPCA61K36/48A61K2236/19A61P17/02A61P29/00
Inventor 萧敬皇
Owner 萧敬皇
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