Lactobacillus salivarius JYLS-372 for improving oral health, product and preparation method thereof

A technology of JYLS-372 and Lactobacillus salivarius, applied in the field of oral care, can solve problems such as endangering the health of the whole body, affecting chewing, speech, aggravating systemic diseases, etc., and achieve the effect of improving oral health, preventing dental caries, and reducing aphthous ulcers

Inactive Publication Date: 2020-02-14
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If oral hygiene is not maintained, it will cause oral diseases such as dental caries, bad breath and aphthous sores. In addition to affecting chewing, speech and other functions, it will also cause soc

Method used

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  • Lactobacillus salivarius JYLS-372 for improving oral health, product and preparation method thereof
  • Lactobacillus salivarius JYLS-372 for improving oral health, product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation and Identification of Lactobacillus salivarius JYLS-372

[0023] 1. Bacterial source

[0024] Qingzhou Xigu breast milk.

[0025] 2. Medium and culture conditions

[0026] (1) Weigh 1 g of the collected sample, place it in a Erlenmeyer flask containing 99 mL of 0.85% sterile saline, shake it for 15 minutes to fully mix the sample, and serially dilute it 10 times to 10 -7 , prepared into bacterial suspension;

[0027] (2) Spread the bacterial suspension on the MRS plate medium, culture at 37°C for 48 hours;

[0028] (3) Pick a single colony of suspected lactic acid bacteria on the MRS plate medium, Gram staining, and microscopic examination;

[0029] (4) Select Gram-positive, rod-shaped single colonies, streak on the MRS plate medium, and culture at 37°C for 48h;

[0030] (5) Repeat the above steps 4 times

[0031] (6) Finally, the single colonies on the plate are cultured on the slant and stored.

[0032] Wherein, the preparation method of MRS...

Embodiment 2

[0034] Embodiment 2 A kind of product that improves oral health

[0035] A product for improving oral health. The active ingredient of the product is Lactobacillus salivarius JYLS-372. The product also contains isomaltooligosaccharide. The content of Lactobacillus salivarius JYLS-372 in the product is 100 billion / g.

[0036] The preparation method of above-mentioned product comprises the following steps:

[0037] (1) Lactobacillus salivarius JYLS-372 was inoculated on the MRS liquid medium and cultured, the inoculation amount was 1%, and the culture condition was 37° C. to obtain the MRS bacterial liquid;

[0038] Wherein, the preparation method of MRS liquid culture medium is:

[0039] Peptone 10g, beef powder 5g, longibacterium powder 4g, K 2 HPO 4 ·7H 2 O 2g, triammonium citrate 2g, sodium acetate 3H 2 O5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.2g and MnSO 4 4H 2 O 0.05g dissolved in 1000mL distilled water;

[0040] (2) The MRS bacterial liquid is centrifuge...

Embodiment 3

[0042] Example 3 In vitro antibacterial effect of Lactobacillus salivarius JYLS-372 on 3 common oral pathogens

[0043] 1. Preparation of indicator bacteria suspension:

[0044] (1) Streptococcus mutans ATCC25175 was streaked and inoculated on the blood agar plate respectively, placed in an anaerobic tank, put into a microaerophilic gas-generating bag, and cultured in a microbial incubator at 35°C for 48 hours, and a single colony was picked and inoculated Place in 10mL BHI in an anaerobic tank, put a bag of microaerophilic gas producing bag, and culture in a microbial incubator at 35°C for 48 hours. After subculture for 3 generations, adjust the concentration of the bacterial solution to 10 6 CFU / mL as the indicator bacteria suspension;

[0045] (2) Porphyromonas gingivalis ATCC33277 was inoculated on a blood agar plate by streaking, placed in an anaerobic tank, put into an anaerobic gas-generating bag, and cultured in a microbial incubator at 35°C for 48 hours. A single col...

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Abstract

The invention relates to the technical field of oral care, in particular to lactobacillus salivarius JYLS-372 for improving oral health, a product and a preparation method thereof, the active ingredient of the product is lactobacillus salivarius JYLS-372, and the preparation method comprises the following steps: inoculating the lactobacillus salivarius JYLS-372 onto an MRS liquid culture medium and culturing to obtain MRS bacterial liquid; carrying out centrifugation and vacuum freeze drying on the MRS bacterial liquid to obtain bacterial powder; and mixing and compounding the bacterial powderand isomaltooligosaccharide to obtain the product. The lactobacillus salivarius JYLS-372 disclosed by the invention can be used for synthesizing various vitamins, such as vitaminB1, vitaminB2, vitaminB12 pantothenic acid and folic acid, required by a human body, and can be used for improving the symptoms of gingival bleeding, tooth decay, halitosis, thrush, ulcer sores and the like; the number ofdecayed tooth pathogenic bacteria mutant streptococcus in the oral cavity is obviously reduced, and the formation of dental plaque and the occurrence rate of periodontal diseases are reduced.

Description

technical field [0001] The invention relates to the technical field of oral care, in particular to a strain of Lactobacillus salivarius JYLS-372 for improving oral health, a product and a preparation method. Background technique [0002] Oral health is a mirror that reflects the quality of life and health. Oral health includes good oral hygiene, sound oral function and no oral diseases, and ensuring oral hygiene is a necessary prerequisite for achieving sound oral functions and avoiding oral diseases. Oral hygiene focuses on controlling plaque, eliminating dirt and debris, and keeping the mouth and teeth clean and healthy. If oral hygiene is not maintained, it will cause oral diseases such as dental caries, bad breath and aphthous sores. In addition to affecting chewing, speech and other functions, it will also cause social difficulties and psychological barriers, and even aggravate certain systemic diseases, such as coronary heart disease and diabetes. Harm the health of t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/02A61P31/04C12R1/225
CPCA61K35/747A61P1/02A61P31/04C12N1/20C12N1/205C12R2001/225
Inventor 司书锋潘玉林李翠华马明磊刘玉江曹维超李青青闫洪伟孙川王维新周文浩
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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