A device and method for improving the DNA detection performance of an optical addressable potential sensor using zno nanorods

Abstract

The invention discloses a device and method for detecting the performance of a DNA by using a ZnO nanorod to enhance an optical addressing potential sensor. The device includes a Faraday shielding box, a loading table, a semiconductor laser, an LAPS sensor, a constant voltage device, a phase-locked amplifier, an acquisition card, and a computer. The method includes the steps of making a LAPS chip,modifying the surface of the LAPS chip with the ZnO nanorod, modifying the surface of the LAPS chip with a probe chain DNA, scanning an I-V curve; placing a target DNA in a detection cavity, scanningthe I-V curve, and comparing the performances of the modified and unmodified ZnO nanorods. Label-free detection of the DNA is realized, and the advantages that the time is short, the cost is low, operation is convenient, three-dimensional sites are provided for DNA hybridization, and sensitivity is high are achieved.

Description

technical field [0001] The invention relates to the field of DNA detection, in particular to a device and method for improving the DNA detection performance of an optical addressing potential sensor by using ZnO nanorods. Background technique [0002] DNA (deoxyribonucleic acid) detection is of great significance in the fields of genetic disease diagnosis, pathogen identification, parental testing, drug screening and food safety. The current method for detecting DNA is PCR amplification technology, labeling method and non-labeling method. The PCR method is to amplify DNA fragments or sequences through enzymatic synthesis reaction, and it takes a long time to amplify a small amount of DNA. The labeling method usually uses fluorescence to label the probe or the target DNA molecule. DNA production costs are high and the detection process is complicated. The non-labeling method generally uses a chip to modify the complementary strand to directly capture the target DNA molecule. ...

AI Technical Summary

Problems solved by technology

The current method of detecting DNA is PCR amplification technology, labeling method and non-labeling method. The PCR method is to amplify DNA fragments or sequences through an enzymatic synthesis reaction, and it takes a long time to amplify a small amount of DNA.

The labeling method usually uses fluorescence to label the probe or the target DNA molecule. DNA production costs are high and the detection process is complicated. The non-labeling method generally uses a chip to modify the complementary strand to directly capture the target DNA molecule. It is simple and fast, but the flat structure may have some effect on DNA hybridization

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