An antibacterial collagen-based bone repair material
A technology for bone repair and collagen, applied in prosthesis, coating, tissue regeneration, etc., can solve the problems of uncontrollable BMP-2 concentration and release time, unsatisfactory repair effect, uncertain porosity, etc., and achieve excellent cell adhesion. Adhesion, promoting cell adhesion and proliferation, high porosity effect
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Embodiment 1
[0033] Preparation of zinc oxide / hydroxyapatite whiskers: Add zinc nitrate and polyethylene glycol 6000 into absolute ethanol, stir, and heat in a constant temperature water bath for 2 hours to dissolve completely to obtain a clear solution; zinc nitrate: polyethylene glycol Weigh zinc nitrate and polyethylene glycol with a mass ratio of 1:2, then weigh an appropriate amount of hydroxyapatite whiskers (HAPw) after drying in a constant temperature drying oven at 80°C for 24 hours, add them together to absolute ethanol solution, and ultrasonically disperse , to obtain the dispersion of HAPw; under magnetic stirring, slowly add the prepared zinc nitrate and polyethylene glycol 6000 clear liquid into the dispersion of HAPw with a digital display constant-current pump, and adjust it by adding ammonia water and glacial acetic acid together. pH, react in a water bath at the set temperature for 6 hours, ultrasonically disperse for 20 seconds after the reaction, heat to 80°C to evaporat...
Embodiment 2
[0035] Preparation of bone repair material: Weigh 15g of PVA, add it to 200mL of distilled water, and heat it in a water bath at 100°C for 20min to prepare a PVA solution with a mass fraction of 7%; weigh 2g of type I collagen and dissolve it in 8mL with a mass fraction of 1 % glacial acetic acid to obtain a type I collagen solution, after the type I collagen is completely dissolved, add 1g ZnO / HAPw to the type I collagen solution, stir evenly to obtain a mixture, add 4mL of the above prepared 7% Stir the PVA solution evenly and put it into a mold, store it overnight at -20°C, and freeze and dry it in vacuum. Dissolve 5.7682g EDC and 1.7314g NHS in 100mL 95% ethanol to obtain a cross-linking agent, take out the lyophilized material and put it in the cross-linking agent for cross-linking for 24h, then take out the material and immerse it in 1M Na 2 HPO 4 Put it in the solution for 2 hours, then take it out and immerse it in 4M NaCl solution, and replace the NaCl solution every...
Embodiment 3
[0037] Amination modification of bone repair material: immerse the bone repair material prepared in Example 2 into 1 mg / mL polyethyleneimine solution for amination modification, take it out after 3 hours, wash it, dry it, and then immerse it in 5% hydrochloric acid After being in the solution for 2 minutes, take it out, wash it, and dry it for later use.
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