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Novel method for diagnosing feline infectious peritonitis

A technology for infectious and peritonitis, which is applied in the field of primer diagnosis of feline coronavirus, can solve the problem of no feline infectious peritonitis, and achieve the effect of improving the accuracy of diagnosis and the determination of sensitive results

Pending Publication Date: 2020-03-24
武汉康湃特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After reviewing the relevant literature, there is no report on the design of this primer and these two amino acid difference sites for the diagnosis of feline infectious peritonitis

Method used

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  • Novel method for diagnosing feline infectious peritonitis
  • Novel method for diagnosing feline infectious peritonitis
  • Novel method for diagnosing feline infectious peritonitis

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Embodiment Construction

[0020] Specific embodiments of the present disclosure will be described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are only used to illustrate and explain the present disclosure, and are not intended to limit the present disclosure.

[0021] The disclosure discloses four sequences: a primer set for feline coronavirus detection, and two amino acid sites for identifying FECV and FIPV. The primer set can diagnose feline coronavirus, and the amino acid difference can be used to distinguish FECV from FIPV. The target band after the primer set amplifies the feline coronavirus is about 1500bp, and this target band includes these two amino acid sequences. Therefore, after the gene sequence is obtained by sequencing, comparison can be made at the two amino acid sequence positions of SEQ ID NO.3-4.

[0022] The present invention also provides a kind of detection method of feline infectious periton...

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Abstract

The invention discloses a novel method for diagnosing feline infectious peritonitis. A kit adopted in the method comprises a primer shown as SEQ ID NO.1-2 and two amino acid differences shown as SEQ ID NO.3-4. The invention further provides a new thought for identifying feline infectious peritonitis (FIPV) and feline enteric corona virus (FECV). By means of the detection scheme, accurate, specificand sensitive result judgment which cannot be completed through an existing detection technology can be achieved, the detection accuracy on feline infectious peritonitis is remarkably improved, and important technical support is provided for discovering and effectively preventing and controlling feline infectious peritonitis as soon as possible.

Description

technical field [0001] The invention belongs to the field of invention, and in particular relates to primers for diagnosing feline coronavirus, and then using the difference of two amino acid sites to identify FECV and FIPV, and the invention belongs to the technical field of virus detection. Background technique [0002] Feline infectious peritonitis (FIP) is a progressive and fatal disease caused by feline infectious peritonitis virus. [0003] At present, domestic diagnosis methods include pathological examination, serological examination and RT-PCR examination. The method of diagnosis based only on clinical symptoms needs to rely on typical clinical symptoms and the experience of the diagnoser, while cats with infectious peritonitis have no obvious initial symptoms, and the progress of the disease varies greatly, resulting in inaccurate results. Delayed treatment due to misdiagnosis eventually led to the death of the sick cat. [0004] Diagnosis is mainly by RT-PCR com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6806C12R1/93
CPCC12Q1/701C12Q1/6806C12Q2531/113C12Q2565/125
Inventor 刘紫微
Owner 武汉康湃特生物科技有限公司
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