Application of escherichia coli freeze-dried powder in testing fumigation disinfection effect

A technology of Escherichia coli, fumigation and disinfection, which is applied in the direction of microbiological determination/inspection, bacteria, and microbiological-based methods. It can solve the problems of increasing labor force and achieve the effects of convenient storage, high activity, and prolonged storage time.

Inactive Publication Date: 2020-03-27
广州迈高化学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has high requirements for experimenters and experiment sites, and it is difficult for most farms to meet the relevant requirements. In addition, in order to detect as accurately as possible, multi-point collection is required, which increases the labor force.

Method used

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  • Application of escherichia coli freeze-dried powder in testing fumigation disinfection effect
  • Application of escherichia coli freeze-dried powder in testing fumigation disinfection effect
  • Application of escherichia coli freeze-dried powder in testing fumigation disinfection effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Reagents and equipment

[0036] (1) The slant culture of the nutrient agar medium of the 3rd to 14th generation of Escherichia coli;

[0037] (2) Medium: nutrient agar medium

[0038] (3) Reagents: distilled water, 0.96% normal saline, skimmed milk powder, sucrose, gelatin

[0039] (4) Diluent: tryptone saline (dissolve 1.0g tryptone and 8.5g sodium chloride in more than 900mL of distilled water, adjust the pH to 7.0, dilute to 1000mL with distilled water, and sterilize by high pressure steam at 121°C).

[0040] 2. Preparation of Escherichia coli freeze-dried powder

[0041] (1) Preparation of Escherichia coli bacterial suspension: Take the slant culture of nutrient agar medium from the 3rd generation to the 14th generation of Escherichia coli, use a 5.0mL pipette to draw 3.0mL dilution liquid into the inclined test tube, blow and suck repeatedly, and then use 5.0 Transfer the washing solution to another sterile test tube with a mL pipette, add 100mL of diluent, a...

Embodiment 2

[0053] A method for testing the effect of fumigation, comprising the following steps:

[0054] (1) Recovery: Take 4g of E. coli freeze-dried powder of Example 1 and add 4mL of sterilized water to make a bacterial liquid, take 4 petri dishes with nutrient agar medium, add 1mL of bacterial liquid to each petri dish , wherein two petri dishes are the experimental group, and the other two petri dishes are the control group;

[0055] (2) Disinfection: place the experimental group in the place to be fumigated, and place the control group in a sterile environment for fumigation;

[0056] (3) Culture: After the fumigation is completed, place the experimental group and the control group in a 37°C incubator for 48 hours;

[0057] (4) Calculation: calculate the colony number of experimental group and matched group after cultivating, and calculate bactericidal rate, calculate bactericidal rate and adopt following formula 1 to calculate:

[0058] η=100% (B-A) / B (formula I);

[0059] Whe...

experiment example 1

[0061] Dilute the Escherichia coli freeze-dried powder of Example 1 with water, and the dilution ratios are 1, 10, 100, 1000, and 10000 times respectively, wherein the standard for 1-fold dilution is to add 1 mL of sterilized water per gram of Escherichia coli freeze-dried powder, dilute 10 times means taking the 1 times diluted solution and then diluting it 10 times, diluting 100 times means taking the 10 times diluted solution and then diluting it 10 times, and so on. Add 1ml of the above bacterial solution to the nutrient agar medium and culture it in a 37°C incubator for 48 hours. The growth of the colony is as follows: figure 2 shown.

[0062] From figure 2 It can be seen that the number of colonies diluted 1 times is too large to be counted, and the number of colonies diluted 10 times is too large to be counted. The number of colonies diluted 100 times is about 300-400 colonies, and the number of colonies diluted 1000 times is about 45, the number of colonies diluted...

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Abstract

The invention provides an application of escherichia coli freeze-dried powder in testing a fumigation disinfection effect, and relates to the technical field of detection methods. The above escherichia coli freeze-dried powder is prepared by the following methods: performing pre-freezing: uniformly mixing an escherichia coli suspension and a freeze-dry stabilizer, and performing pre-freezing; andperforming drying: performing vacuum drying on the pre-frozen escherichia coli to obtain the escherichia coli freeze-dried powder. The escherichia coli freeze-dried powder can quickly, easily and accurately test the disinfection effect of a disinfectant when used to test the fumigation disinfection effect.

Description

technical field [0001] The invention relates to the technical field of detection methods, in particular to the application of Escherichia coli freeze-dried powder in testing the effect of fumigation and disinfection. Background technique [0002] Fumigation is a fast, effective and easy-to-operate disinfection method. The fumigant used in the fumigation method is a molecular gas disinfectant, which has the effect of killing viruses, germs or other harmful organisms. The fumigant can spread to the entire confined space, including some small corners, gaps, etc. All corners of the room are disinfected, truly thoroughly disinfected. After the fumigation and disinfection are completed, open the doors and windows to ventilate and disperse the air to achieve no residue. Fumigation is a disinfection method that is very suitable for breeding sites, and is now more and more accepted by farmers. [0003] At present, there are more and more disinfectants that can be used for fumigati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12Q1/10C12Q1/06C12R1/19
CPCC12N1/20C12N1/04C12Q1/10C12Q1/06G01N2333/245
Inventor 尹曲平李卓鸿冷淑珍李茂亮
Owner 广州迈高化学有限公司
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