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Method for determining concentration of cetirizine in blood plasma by liquid chromatography-mass spectrometry

A technology of cetirizine and LC/MS, applied in the field of medicine, can solve problems such as speed, precision and sensitivity to be improved, and achieve the effects of simple pretreatment method, simple operation and high sensitivity

Inactive Publication Date: 2020-03-27
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the speed, precision and sensitivity of the existing cetirizine determination methods need to be improved

Method used

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  • Method for determining concentration of cetirizine in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of cetirizine in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of cetirizine in blood plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Cetirizine Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Cetirizine (analyte): China National Institutes for Food and Drug Control or the same or higher grade standard Cetirizine-d8 (internal standard): TLC Pharmaceutical Standards or the same or higher grade standard

[0035] The reagents used are shown in Table 1 below:

[0036] Table 1 Reagent Details

[0037] Reagent name level manufacturer Acetonitrile HPLC J. T. Baker Methanol HPLC J. T. Baker formic acid ACS Adamas

[0038] Note: Reagents of the same grade or higher can also be used

[0039] The analytical equipment used is shown in Table 2 below:

[0040] Table 2 Details of equipment used

[0041] components type manufacturer Binary pump (binary pump) AC Pump AB SCIEX Degasser Degasser AB SCIEX Column oven AC Column oven AB SCIEX ...

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Abstract

The invention discloses a method for determining the concentration of cetirizine in blood plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination, and comprises the following steps: taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, performing separating by using a chromatographic column, and performing detecting by using a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of cetirizine; according to the method, the linear range of the plasma standard curve is 1-600ng / mL, the intra-batch precision RSD and the inter-batch precision RSD are both less than + / -15%, and the method is suitable for measuring the concentration of cetirizine in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining the concentration of cetirizine in blood plasma by liquid mass spectrometry. Background technique [0002] The main component of cetirizine is cetirizine hydrochloride, its chemical name is (+ / -)-2-[2-[4-[(4-chlorophenyl)benzyl]-1-piperazinyl ] Ethoxy] acetic acid dihydrochloride, its structural formula is as follows: [0003] [0004] Cetirizine hydrochloride is a new generation of highly effective non-sedating antihistamines for the treatment of seasonal and perennial allergic rhinitis caused by histamine, itchy eyes and skin, chronic idiopathic urticaria, and other antihistamines Compared with amine drugs, the central nervous system activity of cetirizine hydrochloride is lower. At present, there are many dosage forms of cetirizine hydrochloride on the market in China, such as cetirizine hydrochloride dispersible tablets, cetirizine hydrochlorid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/72
CPCG01N30/06G01N30/34G01N30/72G01N30/88G01N2030/045G01N2030/062G01N2030/8822G01N2030/884
Inventor 林明弘
Owner 徐州立兴佳正医药科技有限公司
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