Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of microbial source chitosan

A microbial source, chitosan technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large lye consumption, environmental pollution, etc., reduce the discharge of fermentation waste liquid, reduce alkali Effect of Waste Liquid Discharge

Active Publication Date: 2022-03-18
SHANGHAI INST OF TECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: the existing extraction process of producing chitosan by fermentation of filamentous fungi needs to consume a large amount of lye, which is easy to cause pollution to the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of microbial source chitosan
  • A kind of preparation method of microbial source chitosan
  • A kind of preparation method of microbial source chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Cultivation of Aspergillus ochratum

[0032] Seed medium: 8g of sucrose, 8g of yeast extract, 2.0g of molasses, 1L of water, pH 6.0; sterilize at 121°C for 15 minutes with moist heat, and use after cooling.

[0033] Scrape the yellow spores of the slant strains under aseptic operation and transfer them to sterile normal saline, and adjust the spore concentration to 4.0-7.0×10 8 spores / mL, and then transferred to the sterilized seed medium according to the inoculation amount of 10wt%, and cultivated at 28°C and 180r / min for 24h to obtain the seed liquid.

[0034] Fermentation medium: water 1L, sucrose 30g, yeast extract 20g, soybean powder 8g, molasses 1.5g, K 2 HPO 4 1.5, MgSO 4 ·7H 2 O 2.0g, FeSO 4 ·7H 2 O 0.01g, NaH 2 PO 4 2H 2 O 1.5g, pH value is 5.5; sterilize it at 121°C for 15min with moist heat, and use it after cooling.

[0035] 5L fermenter: the total liquid volume is 3.5L (volume percentage 70%), 350mL seed liquid is aseptically inserted into 315...

Embodiment 2

[0045] (1) Aspergillus ochrae fermented as in Example 1

[0046] (2) Cultivation of Bacillus licheniformis: Cultivation of Bacillus licheniformis: add 200 mL of sterilized nutrient solution to the above fermentation liquid, stir evenly, and adjust the pH of the fermentation liquid to 6.0 with 30% sodium hydroxide solution by mass percentage. The nutrient solution contains 35g of glucose, 3.5g of yeast extract and 7.0g of sodium chloride. The Bacillus licheniformis bacteria powder was inoculated in the medium according to the mass concentration of 10 g / L, and fermented. The culture conditions are: temperature 37° C., rotation speed 150-250 rpm, ventilation rate 0.4-0.7 (v / v) / min, culture time 60 h.

[0047] (3) Collect the filter residue: suction filter the fermentation broth in step (2), take the filter residue, wash the filter residue with water and suction filter, and wash until the filtrate is colorless. The filter residue was dried in a vacuum oven at 45°C and pulverized...

Embodiment 3

[0053] (1) Aspergillus ochrae fermented as in Example 1

[0054] (2) Bacillus licheniformis fermentation culture: add 200 mL of sterilized nutrient solution to the above fermentation liquid, stir evenly, adjust the pH of the fermentation liquid to 7.0 with 30% sodium hydroxide solution. The nutrient solution contains 70g of glucose, 17.5g of yeast extract and 28g of sodium chloride. The Bacillus licheniformis bacteria powder was inoculated in the medium according to the mass concentration of 30 g / L, and fermented. The culture conditions are: temperature 39° C., rotation speed 250-350 rpm, ventilation rate 0.7-1.0 (v / v) / min, and culture time 72 hours.

[0055] (3) Collect the filter residue: suction filter the fermentation broth in step (2), take the filter residue, wash the filter residue with water and suction filter, and wash until the filtrate is colorless. The filter residue was dried in a vacuum oven at 45°C and pulverized in a pulverizer to a particle size of less than...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of chitosan derived from microorganisms, which is characterized in that the mixed fermentation is carried out by using Aspergillus ochratum and Bacillus licheniformis, and then the chitosan is prepared through a deacetylation process; the method comprises the cultivation of Aspergillus ochratum and the cultivation of Bacillus licheniformis , collecting filter residue, acid treatment, deacetylation to prepare chitosan and other steps. In the present invention, the fermentation method is used instead of the alkali method to remove the protein in the ochrax mycelium, and the discharge of alkali waste liquid is effectively reduced; the single-bacteria fermentation of the two-step method is replaced by the one-step mixed-bacteria fermentation, and the discharge of fermentation waste liquid is greatly reduced. The microbe source chitosan prepared by the method of the invention has a miscellaneous protein content of less than 0.10%, an ash content of less than 0.43%, and a deacetylation degree of 91%.

Description

technical field [0001] The invention relates to a preparation process of chitosan, in particular to a method for producing chitosan by mixed fermentation of aspergillus ochratum and bacillus licheniformis, belonging to the technical field of microorganism preparation. Background technique [0002] Chitosan (chitosan), also known as deacetylated chitin, is obtained by deacetylation of chitin (chitin), which exists widely in nature, and its chemical name is polyglucosamine (1-4)-2-amino-glucose. Chitosan is the only alkaline polysaccharide in nature due to the presence of a large number of amino groups in its macromolecular structure, so it has a wide range of application values ​​in the fields of medical treatment, nutrition, health care and cosmetics. [0003] Traditional production of chitosan mainly has two methods: (1) Shrimp and crab shell extraction method. (2) Microbial fermentation method. Compared with the shrimp and crab shell extraction method, the microbial ferm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/26C12N1/20C12N1/14C08B37/08C12R1/10C12R1/66
CPCC12P39/00C12P19/26C12N1/20C12N1/14C08B37/003C08B37/0003
Inventor 管世敏荣绍丰
Owner SHANGHAI INST OF TECH