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Cosmetic composition containing fusion protein with skin penetration enhancing peptide conjugated thereto for skin improvement

A fusion protein, skin penetration technology, applied in the direction of peptide/protein ingredients, preparations for skin care, medical preparations containing active ingredients, etc. To achieve the effect of improving skin retention and improving skin permeability

Pending Publication Date: 2020-04-10
LG HOUSEHOLD & HEALTH CARE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned peptides have the following advantages: not only can show excellent skin permeability, but also can be used as carriers for transdermal delivery of other drugs, but also have the following disadvantages: after penetrating the skin, they are consumed by the circulatory system in the body, so In the case of drugs that target the skin, no special effect can be exhibited
[0006] It is known that the main cause of this disadvantage is that various active ingredients that show wrinkle-improving effects by promoting collagen synthesis, endothelial cell growth, or hyaluronic acid production are generally not absorbed through the skin, so research is actively conducted to try to develop The method of absorption of the above active ingredients
For example, Korean Registered Patent No. 1054519 discloses a human growth hormone-derived peptide having excellent stability and skin permeability compared with natural human growth hormone and a composition containing it, and Korean Registered Patent No. 1104223 discloses a peptide having IL-10-derived peptides having the same function as human IL-10 and having very excellent stability and skin penetration compared to native IL-10 and compositions comprising them, but these peptides have the following disadvantages: only Capable of showing functionality by itself, but cannot be used as a delivery carrier for other drugs
Analysis of this shortcoming suggests that the property of excellent skin permeability alone cannot be used for drug delivery. Therefore, it is a fact that it is necessary to develop a new substance containing both properties that not only has excellent skin permeability but also improves skin retention, but so far So far, no specific research results have been reported

Method used

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  • Cosmetic composition containing fusion protein with skin penetration enhancing peptide conjugated thereto for skin improvement
  • Cosmetic composition containing fusion protein with skin penetration enhancing peptide conjugated thereto for skin improvement
  • Cosmetic composition containing fusion protein with skin penetration enhancing peptide conjugated thereto for skin improvement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] Example 1: Screening of skin penetration enhancing peptides

[0139] In order to screen skin penetration-promoting peptides, a phage display method combining a phage library and a dissolution test method for transdermal preparations was performed.

[0140] First, in 500 mL of TBS (50 mM Tris pH 7.5, 150 mM NaCl) solution containing 1% BSA, 10 9 phages to obtain a phage solution.

[0141] Next, pigskin (0.7mm thick, Medikinetics) was installed between the upper and lower ends of Franz glass cell (standard diameter 9mm, Receiver 5mL, Permgear), and then the above-mentioned phage solution was added to the upper end and reacted for 16 hours to obtain infiltration The above-mentioned pigskin reaches the phage at the receiver at the lower end and amplifies it.

[0142] The above amplification was performed using E. coli ER2738 (New England Biolab) as a host cell. Specifically, 5 mL of the above phage solution was added to Escherichia coli ER2738 strain cultured with shakin...

Embodiment 2

[0145] Example 2: Preparation of a fusion protein in which a peptide for promoting skin penetration is bound to a physiologically active protein

Embodiment 2-1

[0146] Example 2-1: Preparation of a fusion protein in which a peptide for promoting skin penetration is bound to a neurotransmitter release regulating peptide

[0147] Synthesize the fusion protein of SEQ ID NO: 32 comprising the skin penetration promoting peptide having the amino acid sequence of SEQ ID No. 1 obtained from the above-mentioned Example 1 combined with the neurotransmitter release regulating peptide having the amino acid sequence of SEQ ID NO: 2. The fusion protein of SEQ ID NO: 33 combining the peptide for promoting skin penetration with the amino acid sequence of SEQ ID NO: 1 and the neurotransmitter release regulating peptide having the amino acid sequence of SEQ ID NO: The fusion protein of SEQ ID NO: 34 to which the neurotransmitter release-regulating peptide of amino acid sequence No. 4 binds and the peptide for promoting skin penetration having the amino acid sequence of SEQ ID NO: 1 binds to the neurotransmitter release-regulating peptide of botulinum to...

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Abstract

The present invention relates to a cosmetic composition containing a fusion protein with a skin penetration enhancing peptide conjugated thereto for skin improvement and, more specifically, to a fusion protein in which PDGFa is conjugated to a skin penetration enhancing peptide, a cosmetic composition containing the fusion protein for skin improvement, a functional cosmetic product for skin improvement, a cosmetic composition for hair loss relief, and a quasi-drug product composition containing the fusion protein.

Description

technical field [0001] The present invention relates to a cosmetic composition for skin improvement comprising a fusion protein to which a peptide for promoting skin penetration is bound, and more specifically, the present invention relates to a fusion protein comprising a fusion protein which is bound to a peptide for promoting skin penetration and to which a physiologically active protein is bound. The cosmetic composition for skin improvement, the functional cosmetic for skin improvement, the cosmetic composition for hair loss improvement, and the quasi-drug composition comprising the fusion protein. Background technique [0002] Drugs that pass through the skin are used in pain relief patches, smoking cessation patches, pregnancy regulators, etc. due to the convenience of use, and have the purpose of trying to deliver to systemic circulation through the skin. At the same time, there are cases where therapeutic agents for atopy, cosmetics for whitening and wrinkle improve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K14/65A61K8/64A61Q7/00A61Q19/00A61Q19/08A61K38/00C07K14/475C07K14/49
CPCA61K8/64A61K38/00A61Q7/00A61Q19/08C07K7/08C07K14/49C07K14/65A61P17/14C07K2319/00C07K14/52C07K14/57581C07K14/50A61K8/37C07K14/485
Inventor 金镇铉金载润赵浩成尹志善李相和姜来圭李贤钟李雪薰许柔珍金泰润
Owner LG HOUSEHOLD & HEALTH CARE LTD
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