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Method for determining concentration of azithromycin in plasma by liquid chromatography-mass spectrometry

A technology of azithromycin and LC/MS, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, selectivity, etc. to be improved, and achieve the effect of simple pretreatment method, strong specificity and strong specificity

Inactive Publication Date: 2020-04-21
南京立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the speed, precision, sensitivity and selectivity of the existing methods for the determination of azithromycin need to be improved

Method used

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  • Method for determining concentration of azithromycin in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of azithromycin in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of azithromycin in plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Azithromycin Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Azithromycin (analyte): China National Institutes for Food and Drug Control or equivalent or higher grade standard

[0035] Azithromycin- 13 C-d3 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0036] The reagents used are shown in Table 1 below:

[0037] Table 1 Reagent Details

[0038]

[0039]

[0040]Note: Reagents of the same grade or higher can also be used

[0041] The analytical equipment used is shown in Table 2 below:

[0042] Table 2 Details of equipment used

[0043] components model manufacturer Binary pump (binary pump) AC Pump AB SCIEX Degasser Degasser AB SCIEX Column oven AC Column oven AB SCIEX Autosampler (auto sampler) AC Autosampler AB SCIEX Sample rack Rack Changer AB SCIEX Mass spectro...

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PUM

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Abstract

The invention discloses a method for determining the concentration of azithromycin in plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination and comprises the steps of taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction, separating by a chromatographic column after pretreatment,and detecting by a mass spectrum detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the plasma concentration of azithromycin. According to the method, the linear range of a plasma standard curve is 1-500ng / mL, the intra-batch precision RSD and the inter-batch precision RSD are both less than + / -15%, and the method is suitable for determining the concentration of azithromycin in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining a drug, in particular to a method for determining the concentration of azithromycin in blood plasma by liquid mass spectrometry. Background technique [0002] Azithromycin (Azithromycin) belongs to the second generation of macrolide antibiotics, which was successfully developed by Pliva Company of Yugoslavia in the late 1980s. Azithromycin is widely used clinically because of its good tolerance and low adverse reaction rate. [0003] Azithromycin, as a semi-synthetic 15-membered ring lactone antibiotic, is a broad-spectrum antibiotic obtained by modifying the chemical structure of erythromycin. In addition to having antibacterial effects against most bacteria, it is also effective against chlamydia and mycoplasma. activity. The mechanism of action is the same as that of erythromycin. It mainly binds to the 50S subunit of bacterial ribosomes and inhibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/062G01N2030/8813
Inventor 林明弘
Owner 南京立顺康达医药科技有限公司
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