A composite native microbial domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies

A technology for microbial domestication and black and odorous water, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of low content, inadaptability to the water body environment, and inability to become a dominant bacterial group. Achieve the effects of promoting growth metabolism, high positive regulation efficiency, and enhancing biodiversity

Active Publication Date: 2022-07-22
北京京阳环保工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the above-mentioned treatments for black and odorous water bodies are all treated with biological methods, the microbial preparations sprayed are divided into broad-spectrum microorganisms and obligate microorganisms. Broad-spectrum microorganisms include aerobic microorganisms, facultative microorganisms, and anaerobic microorganisms. There are many types of microorganisms, but the content is not large. After being put into the water body, it cannot play a role because it does not adapt to the water body environment or cannot become the dominant bacterial group. It needs a large number of multiple dosages.

Method used

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  • A composite native microbial domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies
  • A composite native microbial domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies

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Embodiment 1

[0050] A composite native microbial domestication enzyme for efficiently improving urban high-pollution black and odorous water bodies, the composite native microbial domestication enzyme is domesticated and obtained through the following steps:

[0051] S1-1: The strains of Clostridium, Bacillus and Methanogen were sampled into the culture solution, the amount of glucose added to the culture solution was 5g / L, anaerobic culture was carried out for 24h, and the culture temperature was 25°C , the content of composite microorganisms in the obtained primary seed liquid is 0.8g / L;

[0052] S1-2: Then inoculate the above-mentioned culture solution into a new culture solution according to the inoculum volume of 6% of the volume for a second anaerobic culture for 72 hours, and the culture temperature is 25°C;

[0053] S1-3: The above-mentioned culture solution is then subjected to cell disruption, followed by membrane filtration, and the cell debris is filtered to obtain a supernatan...

Embodiment 2

[0065] A composite native microbial domestication enzyme for efficiently improving urban highly polluted black and odorous water bodies, the composite native microbial domestication enzyme is domesticated and obtained through the following steps:

[0066] S1-1: The strains of Clostridium, Bacillus and Methanogen were sampled in the culture solution for anaerobic cultivation for 72 hours, the culture temperature was 30°C, and the content of compound microorganisms in the obtained primary seed solution was 1 g / L;

[0067] S1-2: then inoculate the above-mentioned culture solution into a new culture solution according to the volume of 10% of the inoculum for secondary anaerobic culture for 120 hours, and the culture temperature is 30°C;

[0068] S1-3: The above-mentioned culture solution is then subjected to cell disruption, followed by membrane filtration, the pore size of the filter membrane is 0.45um, and the supernatant is obtained after filtering off cell debris;

[0069] S...

Embodiment 3

[0079] A composite native microbial domestication enzyme for efficiently improving urban highly polluted black and odorous water bodies, the composite native microbial domestication enzyme is domesticated and obtained through the following steps:

[0080] S1-1: The strains of Clostridium, Bacillus and Methanogen were sampled in the culture solution for anaerobic cultivation for 48h, the culture temperature was 28°C, and the content of compound microorganisms in the obtained primary seed solution was 0.9 g / L;

[0081] S1-2: then inoculate the above-mentioned culture solution into a new culture solution according to the volume of 8% of the inoculum for secondary anaerobic culture for 96 hours, and the culture temperature is 28°C;

[0082] S1-3: The above-mentioned culture solution is then subjected to cell disruption, followed by membrane filtration, the pore size of the filter membrane is 0.45um, and the supernatant is obtained after filtering off cell debris;

[0083] S1-4: t...

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Abstract

The invention discloses a composite native microorganism domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies. The composite native microorganism domestication enzyme includes acetyl CoA acyltransferase, 1-phosphofructokinase and pyruvate phospho-dikinase. , β-hydroxybutyryl-CoA dehydrogenase, 3-phosphoglycerate kinase, NAD-dependent β-hydroxybutyl-CoA dehydrogenase and thiolase. A method for treating urban black and odorous water bodies includes the following steps: preparing and activating compound native microorganism domestication enzymes; putting the activated compound native microorganism domestication enzymes in black and smelly water bodies for a period of time; and then constructing aquatic plants. The composite indigenous microbial domestication enzyme of the present invention has the advantages of efficiently improving the quality of black and odorous sewage; in addition, the treatment method of the present invention has the advantages of efficiently purifying the water body, and sustainably adjusting and optimizing the water body environment.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, and more particularly, to a compound indigenous microbial domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies. Background technique [0002] Urban rivers are not only channels for urban flood control and drainage, but also an important factor in urban landscape and urban ecological environment. my country's urbanization process is developing rapidly, and rivers have been polluted by pollution sources for a long time, resulting in a decline in water quality. With the intensification of pollution, the self-purification function of water bodies is lost, and urban rivers are black and smelly. Smelly Ditch". [0003] The governance and restoration of the river and lake water environment ecosystem is a long process. Due to the current unsound urban pipe network, the sewage is directly discharged, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12N9/04C12N9/12C12N9/00C02F3/34C12R1/145C12R1/07C12R1/125C12R1/01
CPCC12N9/1029C12N9/1205C12N9/0006C12N9/1217C12N9/1294C12N9/00C02F3/342C12Y203/01009C12Y207/01056C12Y101/01157C12Y207/02003C12Y207/09001
Inventor 霍石磊林绚丽王丽妹钟本帅
Owner 北京京阳环保工程有限公司
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